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E. coli Topoisomerase I Relaxation Activity Inhibition Assay from US Patent US10266550: "Bacterial topoisomerase I inhibitors with antibacterial activity"
Assay data:17 Active, 8 Activity ≤ 1 µM, 26 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMRelated BioAssays by DepositorRelated BioAssays by Target
Inhibition of Escherichia coli topoisomerase 1 mediated DNA relaxation using plasmid pMT-puro DNA as substrate after 30 mins by ethidium bromide staining-based agarose gel electrophoresis method
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of Escherichia coli topoisomerase 1 mediated DNA relaxation at 1 uM using plasmid pMT-puro DNA as substrate after 30 mins by ethidium bromide staining-based agarose gel electrophoresis method
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of Escherichia coli topoisomerase 1 mediated DNA relaxation at 10 uM using plasmid pMT-puro DNA as substrate after 30 mins by ethidium bromide staining-based agarose gel electrophoresis method
Assay data:2 Active, 7 Tested
Poison activity at Escherichia coli Top1 assessed as increase in nickel band intensity at 100 uM using supercoiled pUC19 plasmid DNA as substrate incubated for 30 mins by agarose gel electrophoresis analysis
Poison activity at Escherichia coli Top1 assessed as increase in nickel band intensity at 12.5 to 100 uM using supercoiled pUC19 plasmid DNA as substrate incubated for 30 mins by agarose gel electrophoresis analysis
Inhibition of Escherichia coli Top1 assessed as decrease in relaxation of supercoiled pBR322 plasmid DNA at 100 uM using supercoiled pBR322 plasmid DNA as substrate incubated for 15 mins by agarose gel electrophoresis analysis
Assay data:4 Active, 4 Tested
Inhibition of Escherichia coli topoisomerase 1 assessed as single stranded breaks using pHOT-1 DNA as substrate treated with substrate for 15 mins prior to enzyme addition for 30 mins by agarose gel electrophoresis
Inhibition of Escherichia coli topoisomerase 1-mediated pHOT-1 DNA relaxation at >90 uM treated with pHOT-1 substrate for 15 mins prior to enzyme addition for 30 mins by agarose gel electrophoresis
Inhibition of Escherichia coli topoisomerase 1-mediated pHOT-1 DNA relaxation treated with pHOT-1 substrate for 15 mins prior to enzyme addition for 30 mins by agarose gel electrophoresis
Assay data:2 Tested
Inhibitors of Y. pestis Topo-I using cleavage product accumulation Measured in Biochemical System Using Plate Reader - 2123-01_Inhibitor_Dose_CherryPick_Activity
Assay data:44 Active, 151 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by DepositorRelated BioAssays by Same ProjectRelated BioAssays by Target
Inhibitors of Y. pestis Topo-I using cleavage product accumulation Measured in Biochemical System Using Plate Reader - 2123-01_Inhibitor_SinglePoint_HTS_Activity
Assay data:229 Active, 356500 Tested
SummaryCompounds, ActiveRelated BioAssays by DepositorRelated BioAssays by Same ProjectRelated BioAssays by Target
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