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Inhibition of DNA2 in human MCF7 cells assessed as induction of sensitization to PARP inhibitor MK4827-induced cytotoxicity by measuring synergistic combination index at 2 uM in presence of 1 uM MK4827
Assay data:1 Active, 1 Tested
SummaryCompounds, ActiveRelated BioAssays by Target
Inhibition of DNA2 in HU-treated BRCA2/BODIL depleted in human U2OS cells assessed as induction of nascent DNA degradation by measuring reduction in P-RPA foci at 20 uM
Inhibition of DNA2 in 2 uM CPT-treated human A549 cells assessed as inhibition of DNA replication fork restart at 10 uM pre-incubated for 40 mins followed by incubation with 250 uM C1dU for 40 mins by DNA fiber assay
Inhibition of DNA2 in 150 nM CPT-treated human A549 cells assessed as inhibition of DNA replication fork restart by measuring restarting forks levels at 20 uM pre-incubated for 40 mins followed by incubation with 250 uM C1dU for 40 mins by DNA fiber assay (Rvb = 75%)
Assay data:1 Tested
SummaryRelated BioAssays by Target
Inhibition of DNA2 in human U2OS cells assessed as increase in gammaH2AX expression
Inhibition of DNA2 in human U2OS cells assessed as reduction in homologous recombination pathways by I-Scel/GFP-based reporter gene assay
Inhibition of DNA2 in human U2OS cells assessed as reduction in single-strand annealing pathways by I-Scel/GFP-based reporter gene assay
Inhibition of nuclease activity of flag-tagged recombinant DNA2 (unknown origin) expressed in 293T cells using [32P]-labeled flap DNA substrate incubated for 15 mins by denaturing PAGE method
Binding affinity to flag-tagged recombinant DNA2 L732A mutant (unknown origin) expressed in 293T cells assessed as reduction in DNA2-substrate complex formation using flap DNA substrate by EMSA
Binding affinity to flag-tagged recombinant DNA2 F696A mutant (unknown origin) expressed in 293T cells assessed as reduction in DNA2-substrate complex formation using flap DNA substrate by EMSA
Inhibition of nuclease activity of flag-tagged recombinant DNA2 L732A mutant (unknown origin) expressed in 293T cells using [32P]-labeled flap DNA substrate incubated for 15 mins by denaturing PAGE method
Inhibition of nuclease activity of flag-tagged recombinant DNA2 F696A mutant (unknown origin) expressed in 293T cells using [32P]-labeled flap DNA substrate incubated for 15 mins by denaturing PAGE method
Inhibition of helicase activity of flag-tagged recombinant DNA2 D277A mutant (unknown origin) expressed in 293T cells assessed as remaining 5' to 3' end-dependent DNA helicase activity at 100 mM using M13 phage DNA hybridized to 5'-tailed oligonucleotide substrate and [g-32P]ATP pre-incubated with 15 nM DNA2 enzyme for 1 min followed by substrate addition and measured after 30 mins by native polyacrylamide gel electrophoresis method relative to control
Inhibition of helicase activity of flag-tagged recombinant DNA2 D277A mutant (unknown origin) expressed in 293T cells assessed as remaining 5' to 3' end-dependent DNA helicase activity at 100 mM using M13 phage DNA hybridized to 5'-tailed oligonucleotide substrate and [g-32P]ATP pre-incubated with 10 nM DNA2 enzyme for 1 min followed by substrate addition and measured after 30 mins by native polyacrylamide gel electrophoresis method relative to control
Inhibition of helicase activity of flag-tagged recombinant DNA2 D277A mutant (unknown origin) expressed in 293T cells assessed as remaining 5' to 3' end-dependent DNA helicase activity at 100 mM using M13 phage DNA hybridized to 5'-tailed oligonucleotide substrate and [g-32P]ATP pre-incubated with 5 nM DNA2 enzyme for 1 min followed by substrate addition and measured after 30 mins by native polyacrylamide gel electrophoresis method relative to control
Binding affinity to flag-tagged recombinant DNA2 (unknown origin) expressed in 293T cells assessed as reduction in DNA2-substrate complex formation using flap DNA substrate by EMSA
Binding affinity to flag-tagged recombinant DNA2 (unknown origin) expressed in 293T cells assessed as reduction in DNA2-substrate complex formation at 1 to 1000 uM using flap DNA substrate by EMSA
Inhibition of DNA dependent ATPase activity of flag-tagged recombinant DNA2 D294A mutant (unknown origin) expressed in 293T cells at 1 to 100 uM using flap DNA substrate
Competitive inhibition of nuclease activity of flag-tagged recombinant DNA2 (unknown origin) expressed in 293T cells using [32P]-labeled flap DNA substrate incubated for 15 mins by denaturing PAGE method based Lineweaver-Burk plot analysis
Inhibition of DNA dependent ATPase activity of flag-tagged recombinant DNA2 D294A mutant (unknown origin) expressed in 293T cells using flap DNA substrate
Assay data:5 Active, 38 Tested
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