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Inhibition of Plasmodium falciparum recombinant His-tagged falcipain-2 expressed in Escherichia coli assessed as inhibition constant using Cbz-Phe-Arg-AMC as substrate by fluorescence spectrophotometer assay
Assay data:2 Active, 2 Tested
SummaryRelated BioAssays by Target
Inhibition of Plasmodium falciparum FP2 using Z-Leu-Arg-AMC as substrate incubated for 30 mins by spectrofluorometry analysis
Assay data:5 Active, 1 Activity ≤ 1 µM, 8 Tested
Inhibition of recombinant Plasmodium falciparum 3D7 FP2
Assay data:2 Tested
Inhibition of Plasmodium falciparum trophozite extracts FP-2
Assay data:3 Active, 2 Activity ≤ 1 nM, 3 Activity ≤ 1 µM, 3 Tested
Inhibition of Plasmodium falciparum FP-2 using Cbz-Leu-Arg-AMC as substrate and measured for 5 to 10 mins by fluorescence spectrophotometric analysis
Assay data:2 Active, 1 Activity ≤ 1 µM, 2 Tested
Binding affinity to Plasmodium falciparum FP-2 by surface plasmon resonance analysis
Assay data:1 Tested
Inhibition of Plasmodium falciparum FP-2 using Z-Phe-Arg-AMC as substrate pretreated with enzyme for 10 mins followed by susbtrate addition and measured after 10 mins by fluorescence based analysis
Inhibition of Plasmodium falciparum FP-2 using Cbz-Phe-Arg-AMC as substrate pretreated with enzyme for 10 mins followed by substrate addition and measured after 10 mins by fluorescence based analysis
Assay data:1 Active, 5 Tested
Inhibition of Plasmodium falciparum FP-2 using Z-Leu-Arg-AMC as substrate pretreated with enzyme for 30 mins followed by substrate addition and measured for 30 mins by spectrofluorometry
Assay data:14 Active, 6 Activity ≤ 1 µM, 18 Tested
Inhibition of Plasmodium falciparum FP-2 using Z-Leu-Arg-AMC as substrate at 10 uM pretreated with enzyme for 30 mins followed by substrate addition and measured for 30 mins by spectrofluorometry
Assay data:9 Tested
Inhibition of Plasmodium falciparum FP-2 using Z-Leu-Arg-AMC as substrate pretreated with enzyme for 30 mins followed by substrate addition and measured for 15 mins by spectrofluorometry
Inhibition of Plasmodium falciparum FP2 using ZFR-AMC as substrate preincubated with enzyme for 10 mins followed by substrate addition and measured for 30 mins by fluorimetric analysis
Assay data:5 Active, 13 Tested
Inhibition of Plasmodium falciparum FP-2 using Z-phe-Arg-AMC as substrate pretreated with enzyme for 30 mins followed by substrate addition and measured for 30 mins by fluorescence based analysis
Inhibition of Plasmodium falciparum D10 trophozite lysates FP-2 pretreated with compound for 1 hr followed by labeling with Cy5-DCG04 for 1 hr by fluorescence scanning analysis
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
Inhibition of Plasmodium falciparum Itg2 trophozite extracts FP-2 using Z-phe-Arg-AMC as substrate pretreated with enzyme for 30 mins followed by substrate addition and measured for 30 mins by spectrofluorometry
Assay data:7 Active, 4 Activity ≤ 1 µM, 7 Tested
Inhibition of recombinant Plasmodium falciparum falcipain-2 expressed in Escherichia coli M15 assessed as reduction in free AMC release using Z-FR-AMC as a substrate measured over 30 mins by Cheng-prusoff equation analysis
Assay data:8 Active, 20 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of recombinant Plasmodium falciparum falcipain-2 expressed in Escherichia coli M15 assessed as reduction in free AMC release using Z-FR-AMC as a substrate measured over 30 mins by fluorometric assay
Assay data:7 Active, 20 Tested
Inhibition of Plasmodium falciparum FP-2
Assay data:1 Active, 1 Tested
Inhibition of recombinant Plasmodium falciparum falcipain-2 expressed in bacterial expression system using ZFR-AMC as substrate measured for 30 mins by fluorimetric assay
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of recombinant Plasmodium falciparum falcipain-2 expressed in bacterial expression system using ZFR-AMC as substrate at 100 uM measured for 30 mins by fluorimetric assay relative to control
Assay data:17 Tested
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