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PKMYT1 SGC Tm panel (DSF)
Assay data:1 Tested
SummaryRelated BioAssays by Target
Thermal Shift Assay. Domain: start/stop: H75-P362
Assay data:27 Tested
Inhibition of full length Nano-luc fused PKMYT1 in human HEK-293T cells incubated for 2 hrs by cell based NanoBRET target engagement assay
Assay data:9 Active, 3 Activity ≤ 1 nM, 9 Activity ≤ 1 µM, 9 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of N-terminal recombinant PKMYT1 (76 to 362 residues) in CCNE1 amplified human FU-OV-1 cells assessed as phosphorylation of CDK1 at Thr14 incubated for 2 hrs by AlphaLisa assay
Assay data:33 Active, 31 Activity ≤ 1 µM, 35 Tested
Inhibition of N-terminal human recombinant PKMYT1 (76 to 362 residues) expressed in Escherichia coli (DE3) RIL preincubated with compound for 15 mins in presence of ATP followed by 1 hr incubation by ADP-glo luminescence assay
Assay data:31 Active, 26 Activity ≤ 1 µM, 38 Tested
PKMYT1 DiscoveRx kinase panel
HotSpot Kinase Assay from US Patent US11332473: "Substituted pyrazolo[3,4-d]pyrimidines as Wee1 inhibitors"
Assay data:17 Active, 4 Activity ≤ 1 µM, 26 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMRelated BioAssays by DepositorRelated BioAssays by Target
KinomeScan assay: inhibition of PKMYT1
Assay data:108 Tested
PKMYT1 Invitrogen Lab (UK) kinase screen (BI)
Inhibition of PKMYT1 (unknown origin) assessed as residual activity measured at 1 uM
Assay data:3 Tested
Inhibition of PKMYT1 (unknown origin) using Myelin basic protein substrate preincubated with enzyme and substrate for 20 mins followed by [33P]ATP and ATP addition and measured after 2 hrs by HotSpot Kinase assay
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMRelated BioAssays by Target
Inhibition of human PKMYT1 assessed as percent of control at 2000 nM by KINOMEscan assay relative to control
Inhibition of DNA-tagged human PKMYT1 assessed as percent of control at 50000 nM by qPCR analysis relative to control
Inhibition of human PKMYT1 assessed as percent of control at 1000 nM by KINOMEscan assay relative to control
Inhibition of human partial length PKMYT1 (A88 to G366 residues) expressed in bacterial expression system assessed as residual activity at 10 uM by kinome scan method relative to control
SummaryPubMed CitationRelated BioAssays by Target
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