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Biological Example 1: Bcl-2 Competition Binding (Fluorescence Polarization) Assay from US Patent US20240166646: "1H-PYRROLO[2,3-B]PYRIDINE DERIVATIVES AS BCL-2 INHIBITORS FOR THE TREATMENT OF NEOPLASTIC AND AUTOIMMUNE DISEASES"
Assay data:60 Active, 49 Activity ≤ 1 nM, 60 Activity ≤ 1 µM, 60 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMRelated BioAssays by Target
Time Resolved-Fluorescence Resonance Energy Transfer (TR-FRET) Assay from US Patent US11964990: "1,3,4,7-tetrahydro-2H-pyrrolo[3′,2′:5,6]pyrido[2,3-b][1,4]oxazepine bcl-2 inhibitors"
Assay data:50 Active, 46 Activity ≤ 1 nM, 50 Activity ≤ 1 µM, 50 Tested
Time Resolved-Fluorescence Resonance Energy Transfer (TR-FRET) Assay from US Patent US20240043404: "APOPTOSIS INDUCING AGENTS FOR THE TREATMENT OF CANCER AND IMMUNE AND AUTOIMMUNE DISEASES"
Assay data:372 Active, 356 Activity ≤ 1 nM, 372 Activity ≤ 1 µM, 372 Tested
HTRF (BCL2)
Assay data:1 Tested
SummaryRelated BioAssays by Target
Inhibition of his-tagged human recombinant Bcl-2 expressed in Escherichia coli /human Bim measured for 60 mins by by TR-FRET based LanthaScreen Eu-Kinase binding assay
Assay data:29 Active, 28 Activity ≤ 1 µM, 29 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of BCL-2 (unknown origin) using FITC-Ahx-GQVGRQLAIIGDDINR-CONH2 as substrate incubated for 4 hrs by fluorescence polarization competition assay
Assay data:5 Active, 5 Activity ≤ 1 µM, 5 Tested
Inhibition of fluorescent-labeled BID-BH3 peptide binding to Bcl-2 (unknown origin) by fluorescence polarization assay
Assay data:2 Active, 2 Activity ≤ 1 µM, 2 Tested
Inhibition of Bcl-2 (unknown origin) by fluorescence polarization assay
Assay data:2 Active, 1 Activity ≤ 1 µM, 2 Tested
Displacement of fluorescein tagged BID BH3 peptide from GST-tagged recombinant Bcl-2 (unknown origin) by fluorescence polarization-based competitive binding assay
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of Bcl-2 (unknown origin)
Inhibition of Bcl-2 (unknown origin) by ELISA analysis
Binding affinity to recombinant Bcl-2 (unknown origin) expressed in Escherichia coli BL21 (DE3) cells assessed as dissociation constant by MST analysis
SummaryPubMed CitationRelated BioAssays by Target
Binding affinity to Bcl-2 (unknown origin) by ELISA analysis
Binding affinity to Bcl-2 (unknown origin) assessed as inhibition constant by TR-FRET assay
Assay data:1 Active, 1 Activity ≤ 1 nM, 1 Activity ≤ 1 µM, 1 Tested
Binding affinity to Bcl-2 (unknown origin) assessed as inhibition constant by competitive fluorescence polarization assay
Assay data:2 Activity ≤ 1 nM, 2 Activity ≤ 1 µM, 2 Tested
SummaryCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Binding affinity to Bcl-2 (unknown origin) assessed as inhibition constant
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
Inhibition of Flu-BakBH3 binding to GST-tagged Bcl-2 (unknown origin) preincubated for 10 mins followed by Flu-BakBH3 addition and measured after 30 mins by competitive fluorescence polarization assay
Inhibition of BH3 peptide binding to Bcl-2 (unknown origin)
Inhibition of Flu-BakBH3 binding to Bcl-2 (unknown origin) assessed as inhibition constant by competitive fluorescence polarization assay
Inhibition of Flu-BakBH3 binding to recombinant GST-tagged Bcl-2 (unknown origin) incubated for 30 mins by competitive fluorescence polarization assay
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