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HTRF (BCL2A1)
Assay data:1 Tested
SummaryRelated BioAssays by Target
Displacement of fluorescein tagged BID BH3 peptide from GST-tagged recombinant BFL-1 (unknown origin) by fluorescence polarization-based competitive binding assay
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Displacement of fluorescent-labeled BID-BH3 peptide from His-tagged BFL-1 (unknown origin)
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of Bfl-1 (unknown origin) by fluorescence polarization assay
Assay data:6 Active, 4 Activity ≤ 1 µM, 6 Tested
Inhibition of Bfl-1/Bim (unknown origin) incubated for 30 mins by TR-FRET assay
Assay data:2 Active, 2 Tested
Inhibition of Bfl-1/Bim (unknown origin) assessed as inhibition constant by fluorescence polarization assay
Assay data:2 Active, 1 Activity ≤ 1 µM, 2 Tested
Inhibition of Bfl-1/Bim (unknown origin) by fluorescence polarization assay
Inhibition of C-terminal GST-tagged Bfl-1 (unknown origin) incubated for 2 hrs by TR-FRET assay
Inhibition of C-terminal GST-tagged Bfl-1 (unknown origin) incubated for 2 hrs by fluorescence polarization assay
Inhibition of BH3 peptide binding to Bfl-1 (unknown origin)
Inhibition of human BFL-1/PUMA protein-protein interaction in human Kasumi 1 cells assessed as decrease in PUMA protein level at 80 uM measured after 5 hrs by co-immunoprecipitation assay
Inhibition of human BFL-1/PUMA protein-protein interaction in human Kasumi 1 cells assessed as increase in BFL-1 protein level at 20 to 80 uM measured after 5 hrs by co-immunoprecipitation assay
Binding affinity to His-tagged recombinant BFL-1 (1 to 151 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) assessed as dissociation constant by surface plasmon resonance analysis
Binding affinity to His-tagged recombinant BFL-1 (1 to 151 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) assessed as association constant by surface plasmon resonance analysis
SummaryPubMed CitationRelated BioAssays by Target
Displacement of fluorescein tagged BID BH3 peptide from His-tagged recombinant BFL-1 (1 to 151 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) assessed as inhibition constant incubated for 24 hrs by fluorescence polarization-based competitive binding assay
Assay data:8 Active, 2 Activity ≤ 1 µM, 19 Tested
Inhibition of His-tagged human BFL-1(1 to 151 residues) expressed in Escherichia coli BL21 (DE3) by fluorescence polarization-based binding assay
Displacement of fluorescent-labeled BID-BH3 peptide from His-tagged human BFL-1 (1 to 151 residues) expressed in Escherichia coli BL21 (DE3) assessed as inhibition constant by fluorescence polarization-based competitive binding assay
Assay data:3 Active, 2 Activity ≤ 1 µM, 3 Tested
Binding affinity to Bfl-1/Bid (unknown origin) incubated for 3 hrs by fluorescence polarization-based binding assay
Inhibition of Bfl-1 (unknown origin)/ human Bid by fluorescence polarization assay
Inhibition of Bfl-1/Bid (unknown origin) by fluorescence polarization assay
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