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Inhibition of ERK5 (unknown origin) at 10 uM by biochemical hotspot kinase assay
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Selectivity interaction (Kinase panel (ATP-site competition binding assay)) EUB0000122b MAPK7
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMRelated BioAssays by Target
Affinity Biochemical interaction (TR-FRET assay (250 M ATP)) EUB0000708a MAPK7
SummaryRelated BioAssays by Target
Affinity Biochemical interaction (TR-FRET assay (250 M ATP)) EUB0000250b MAPK7
Affinity Biochemical interaction (Radiometric assay) EUB0000687a MAPK7
Affinity Biochemical interaction (ATP-binding displacement assay) EUB0000122b MAPK7
Affinity On-target Cellular interaction (Cellular luciferase reporter assay (using the SN12C-MEF2-luc cell line and ONE-Glo)) EUB0000708a MAPK7
Affinity On-target Cellular interaction (Cellular luciferase reporter assay (using the SN12C-MEF2-luc cell line and ONE-Glo)) EUB0000250b MAPK7
Affinity On-target Cellular interaction (EGF-stimulated autophosphorylation of ERK5 in HeLa cells) EUB0000687a MAPK7
Affinity On-target Cellular interaction (KiNativ assay (HeLa cell lysate)) EUB0000122b MAPK7
Assay data:1 Active, 1 Tested
SummaryCompounds, ActiveRelated BioAssays by Target
Affinity Phenotypic Cellular interaction (Western Blot (inhibition of anti-autophosphorylation in HeLa cells)) EUB0000687a MAPK7
Affinity Phenotypic Cellular interaction (Reduction of BMK1-dependent transactivating activity of MEF2C) EUB0000122b MAPK7
Inhibition of human recombinant N-terminal His-tagged / TEV cleavage site fused ERK5 (2 to 409 residues) expressed in baculovirus infected Sf9 cells using K-5FAM-LVEPLTPSGEAPNQ-COOH as substrate incubated for 2 hrs in presence of ATP by IMAP-FP assay
Assay data:2 Active, 2 Activity ≤ 1 µM, 2 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of human recombinant N-terminal GST-tagged ERK5 (1 to 398 residues) expressed in Escherichia coli using biotinylated peptide Ahx-PPGDYSTTPGGTLFSTTPGGTRI as substrate preincubated for 15 mins followed by substrate addition measured after 60 mins in presence of ATP by TR-FRET method
Inhibition of ERK5 (unknown origin) incubated for 1 hr by KiNativ profiling analysis
Inhibition of ERK5 (unknown origin) expressed in baculovirus expression system incubated for 90 mins in presence of ATP by PKlight ATP detection reagent based luciferase assay
Inhibition of ERK5 (1 to 492 residues) in HEK293 cells assessed as paradoxical activation of ERK5 transcriptional activity at 0.3 uM incubated for 20 hrs by dual luciferase reporter assay relative to control
Inhibition of full-length ERK5 in HEK293 cells assessed as paradoxical activation of ERK5 transcriptional activity incubated for 20 hrs by dual luciferase reporter assay
Inhibition of ERK5 (1 to 492 residues) in HEK293 cells assessed as paradoxical activation of ERK5 transcriptional activity incubated for 20 hrs by dual luciferase reporter assay
Binding affinity to wild-type human partial length MAPK7 (M1 to A409 residues) expressed in bacterial expression system by Kinomescan assay
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