Warning: The NCBI web site requires JavaScript to function. more...
An official website of the United States government
The .gov means it's official. Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.
The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.
Inhibition of 6his tagged West Nile virus NS2B-NS3 protease expressed in Escherichia coli BL21 CodonPlus (DE3)-RIPL cells using Pyr-RTKR-AMC as substrate assessed as substrate cleavage preincubated for 30 mins followed by substrate addition by fluorescence spectrophotometric analysis
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of West Nile virus NS2B-NS3 protease expressed in Escherichia coli BL21 (DE3) cells using Bz-Nle-K-K-R-AM as substrate preincubated for 10 mins followed by substrate addition and measured after 60 mins by fluorescence based assay
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of recombinant West Nile virus NS2B-NS3 protease using Pyr-RTKR-AMC as substrate assessed as change in fluorescence intensity preincubated for 15 mins followed by substrate addition by fluorescence based microplate reader analysis
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Uncompetitive inhibition of recombinant West Nile virus NS2B-NS3 protease using Pyr-RTKR-AMC as substrate assessed as inhibition constant preincubated for 15 mins followed by substrate addition
Assay data:2 Active, 2 Tested
Inhibition of West Nile virus NS2B (49 to 96 residues)-NS3 (2 to 184 residues) protease expressed in Escherichia coli BL21-rosetta cells using Bz-Nle-Lys-Lys-Arg-AMC as substrate preincubated for 10 mins followed by substrate addition and measured every 30 secs
Inhibition of recombinant West Nile virus NS2B-NS3 protease using pERTKR-AMC as substrate assessed as inhibition constant by fluorescence based assay
Inhibition of West Nile virus NS2B-NS3 protease using Boc-Gly-Lys-Arg-7-amino-4-methyl coumarin as substrate preincubated for 15 mins followed by substrate addition and measured after 15 mins by fluorescence based assay
Inhibition of West Nile virus NS2B-NS3 protease using Bz-nKRR-AMC as substrate assessed as inhibition constant preincubated for 30 mins followed by substrate addition and measured after 60 mins by fluorescence based microplate reader analysis
Inhibition of West Nile virus NS2B-NS3 protease
Inhibition of West Nile virus NS2B-NS3 protease assessed as dissociation constant by NMR spectroscopic anlaysis
Inhibition of West Nile virus NS2B-NS3 protease expressed in Escherichia coli using Phe-Ala-Ser-Gly-Lys-Arg-pNA assessed as proteolytic cleavage of the substrate by FRET-based assay
Inhibition of West Nile virus NS2B-NS3 protease expressed in Escherichia coli using Bz-Nle-Lys-Arg-Arg-AMC as substrate assessed as proteolytic activity by monochrometer-based spectrofluorometeric analysis
Inhibition of West Nile virus NS2B (1393 to 1440 residues)-NS3 (1476 to 1687 residues) protease expressed in Escherichia coli BL21 (DE3) codon plus cells using Pyr-RTKR-AMC as substrate preincubated for 30 mins followed by substrate addition and measured after 90 mins by fluorescence spectrophotometric analysis
Inhibition of West Nile virus NS2B-NS3 protease using PhAc-Leu-LysLys-Arg-AMC as substrate measured for 10 mins by fluorescence plate reader analysis
Inhibition of West Nile virus NS2B (52 to 96 residues)-NS3 (1 to 184 residues) protease using PhAc-Leu-LysLys-Arg-AMC3 TFA as substrate by fluorescence plate reader analysis
Inhibition of West Nile virus NS2B-NS3 protease using Pyr-RTKR-AMC as substrate preincubated for 10 mins followed by substrate addition and measured after 30 mins by fluorescence based microplate analysis
Inhibition of West Nile virus NS2B-NS3 protease expressed in Escherichia coli BL21 (DE3) RILP codon plus competent cells using Benzoyl-NleKRR-AMC as substrate preincubated for 15 mins followed by substrate addition and measured after 30 mins by fluorescence based microplate reader analysis
Mixed type inhibition of West Nile virus NSB2-NS3 protease using Abz-Nle-Lys-Arg-Arg-Ser-3-(NO2)Tyr as substrate preincubated with compound for 15 mins followed by substrate addition and measured after 15 mins by fluorescence based microtiter plate reader analysis
Competitive inhibition of C-terminal His-tagged WNV NSB2 (52 to 96 amino acids)-NS3 (1 to 184 amino acids) protease expressed in Escherichia coli BL21(DE3) using Boc-GRK-AMC as substrate measured by flourescence based plate reader assay
Inhibition of C-terminal His-tagged WNV NSB2 (52 to 96 amino acids)-NS3 (1 to 184 amino acids) protease expressed in Escherichia coli BL21(DE3) using Boc-GRK-AMC as substrate measured by flourescence based plate reader assay
Filters: Manage Filters
Your browsing activity is empty.
Activity recording is turned off.
Turn recording back on