Protein Family Models

The alpha subunit of RNA polymerase consists of two independently folded domains, referred to as amino-terminal and carboxyl terminal domains. The amino terminal domain is involved in the interaction with the other subunits of the RNA polymerase. The carboxyl-terminal domain interacts with the DNA and activators. The amino acid sequence of the alpha subunit is conserved in prokaryotic and chloroplast RNA polymerases. There are three regions of particularly strong conservation, two in the amino-terminal and one in the carboxyl- terminal [2]. NMR structure. [1]. 7491496. Solution structure of the activator contact domain of the RNA. polymerase alpha subunit.. Jeon YH, Negishi T, Shirakawa M, Yamazaki T, Fujita N, Ishihama. A, Kyogoku Y;. Science 1995;270:1495-1497.. [2]. 7613089. The Escherichia coli RNA polymerase alpha subunit: structure and. function.. Ebright RH, Busby S;. Curr Opin Genet Dev 1995;5:197-203.. [3]. 9050843. The two alpha subunits of Escherichia coli RNA polymerase are. asymmetrically arranged and contact different halves of the DNA. upstream element.. Murakami K, Kimura M, Owens JT, Meares CF, Ishihama A;. Proc Natl Acad Sci USA 1997;94:1709-1714. (from Pfam)

Date:

2024-08-14

Members of this family include: alpha subunit from eubacteria alpha subunits from chloroplasts Rpb3 subunits from eukaryotes RpoD subunits from archaeal [1]. 9657722. Structure of the Escherichia coli RNA polymerase alpha subunit. amino-terminal domain.. Zhang G, Darst SA;. Science 1998;281:262-266.. [2]. 7613089. The Escherichia coli RNA polymerase alpha subunit: structure and. function.. Ebright RH, Busby S;. Curr Opin Genet Dev 1995;5:197-203.. [3]. 9050843. The two alpha subunits of Escherichia coli RNA polymerase are. asymmetrically arranged and contact different halves of the DNA. upstream element.. Murakami K, Kimura M, Owens JT, Meares CF, Ishihama A;. Proc Natl Acad Sci USA 1997;94:1709-1714. (from Pfam)

Date:

2024-08-14

The two eukaryotic subunits Rpb3 and Rpb11 dimerise to from a platform onto which the other subunits of the RNA polymerase assemble (D/L in archaea). The prokaryotic equivalent of the Rpb3/Rpb11 platform is the alpha-alpha dimer. The dimerisation domain of the alpha subunit/Rpb3 is interrupted by an insert domain (Pfam:PF01000). Some of the alpha subunits also contain iron-sulphur binding domains (Pfam:PF00037). Rpb11 is found as a continuous domain. Members of this family include: alpha subunit from eubacteria, alpha subunits from chloroplasts, Rpb3 subunits from eukaryotes, Rpb11 subunits from eukaryotes, RpoD subunits from archaeal spp, and RpoL subunits from archaeal spp. [1]. 9657722. Structure of the Escherichia coli RNA polymerase alpha subunit. amino-terminal domain.. Zhang G, Darst SA;. Science 1998;281:262-266.. [2]. 7613089. The Escherichia coli RNA polymerase alpha subunit: structure and. function.. Ebright RH, Busby S;. Curr Opin Genet Dev 1995;5:197-203.. [3]. 9050843. The two alpha subunits of Escherichia coli RNA polymerase are. asymmetrically arranged and contact different halves of the DNA. upstream element.. Murakami K, Kimura M, Owens JT, Meares CF, Ishihama A;. Proc Natl Acad Sci USA 1997;94:1709-1714. (from Pfam)

Date:

2024-08-14

DNA-directed RNA polymerase subunit alpha

Date:

2023-03-08

Gene:

rpoA

Date:

2021-08-30

Date:

2022-02-09

Date:

2021-09-15

Date:

2021-08-30

This family consists of the bacterial (and chloroplast) DNA-directed RNA polymerase alpha subunit, encoded by the rpoA gene. The RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The amino terminal domain is involved in dimerizing and assembling the other RNA polymerase subunits into a transcriptionally active enzyme. The carboxy-terminal domain contains determinants for interaction with DNA and with transcriptional activator proteins.

Gene:

rpoA

Date:

2024-05-14