Protein Family Models

The central intermediate formed during mitotic and meiotic recombination is a four stranded DNA structure, also known as the Holliday junction (HJ), and its efficient resolution is essential for proper segregation of chromosomes. Resolution of HJs is mediated by a diverse group of DNA structure specific endonucleases known as Holliday junction resolvases (HJR) [1]. This entry is specific for RuvX also known as YqgF a family of nucleases which resolves the Holliday junction intermediates in genetic recombination[2-3]. Studies carried out in M. tuberculosis, have shown that YqgF/RuvX is a genuine HJR analogous to RuvC from E. coli. Furthermore, a single cysteine present in M. tuberculosis RuvX was found to be required for disulfide-bond mediated intermolecular dimerization and HJ resolution activity, suggesting that M. tuberculosis RuvX has adapted its YqgF protein to function like a typical RuvC family HJR [1]. [1]. 26817626. Mycobacterium tuberculosis RuvX is a Holliday junction resolvase. formed by dimerisation of the monomeric YqgF nuclease domain.. Nautiyal A, Rani PS, Sharples GJ, Muniyappa K;. Mol Microbiol. 2016;100:656-674.. [2]. 10982859. Holliday junction resolvases and related nucleases:. identification of new families, phyletic distribution and. evolutionary trajectories.. Aravind L, Makarova KS, Koonin EV;. Nucleic Acids Res. 2000;28:3417-3432.. [3]. 1661673. Escherichia coli RuvC protein is an endonuclease that resolves. the Holliday structure.. Iwasaki H, Takahagi M, Shiba T, Nakata A, Shinagawa H;. EMBO J. 1991;10:4381-4389. (from Pfam)

Gene:

ruvX

Date:

2024-07-08

RuvX/YqgF family protein such as Holliday junction resolvase RuvX, which acts as a dimer, or the monomeric pre-16S rRNA-processing nuclease YqgF, which was shown to process the 5' end of pre-16S rRNA within 70S ribosomes in vitro

Date:

2021-01-12

RuvX/YqgF family protein such as Holliday junction resolvase RuvX, which acts as a dimer, or the monomeric pre-16S rRNA-processing nuclease YqgF, which was shown to process the 5' end of pre-16S rRNA within 70S ribosomes in vitro

Date:

2021-01-12

This protein family, which exhibits an RNAse H fold in crystal structure, has been proposed as a putative Holliday junction resolvase, an alternate to RuvC, but also has been proposed as a pre-16S rRNA nuclease.

Gene:

ruvX

Date:

2024-05-29