Protein Family Models

Initiation of packaging of double-stranded viral DNA involves the specific interaction of the prohead with viral DNA in a process mediated by a phage-encoded terminase protein. The terminase enzymes are usually hetero-oligomers composed of a small and a large subunit. This region is found on the large subunit and possess an endonuclease and ATPase activity that require Mg2+ and a neutral or slightly basic reaction. This region is also found in bacterial sequences [1,2]. [1]. 10930407. Functional analysis of the terminase large subunit, G2P, of. Bacillus subtilis bacteriophage SPP1.. Gual A, Camacho AG, Alonso JC;. J Biol Chem 2000;275:35311-35319.. [2]. 1548711. Molecular analysis of the Bacillus subtilis bacteriophage SPP1. region encompassing genes 1 to 6. The products of gene 1 and. gene 2 are required for pac cleavage.. Chai S, Bravo A, Luder G, Nedlin A, Trautner TA, Alonso JC;. J Mol Biol 1992;224:87-102. (from Pfam)

Date:

2024-08-14

This entry represents the N-terminal domain of terminase large subunits found in a variety of the Caudovirales and prophage regions of bacterial genomes. It includes the terminase large subunit of Bacteriophage T4 (terminase gene 17, Gp17) [1,3]. Homologues are also found in Gene Transfer Agents (GTA) [2], including ORFg2 (RCAP_rcc01683) of the GTA of Rhodobacter capsulatus (Rhodopseudomonas capsulata) [see Fig.1, in 4]. [1]. 12235385. Sequence analysis of bacteriophage T4 DNA packaging/terminase. genes 16 and 17 reveals a common ATPase center in the large. subunit of viral terminases.. Mitchell MS, Matsuzaki S, Imai S, Rao VB;. Nucleic Acids Res. 2002;30:4009-4021.. [2]. 11382219. The gene transfer agent of Rhodobacter capsulatus and. "constitutive transduction" in prokaryotes.. Lang AS, Beatty JT;. Arch Microbiol. 2001;175:241-249.. [3]. 12466275. Isolation and characterization of T4 bacteriophage gp17. terminase, a large subunit multimer with enhanced ATPase. activity.. Baumann RG, Black LW;. J Biol Chem. 2003;278:4618-4627.. [4]. 12399927. Evolutionary implications of phylogenetic analyses of the gene. transfer agent (GTA) of Rhodobacter capsulatus.. Lang AS, Taylor TA, Beatty JT;. J Mol Evol. 2002;55:534-543. (from Pfam)

Date:

2024-08-14