Various T7 RNA polymerase-based RNA amplification/labeling methods
Summary:
Comparison of expression profiles generated using various T7 RNA polymerase-based in vitro transcription (IVT) RNA labeling methods. The length of the IVT reactions ranged from 4 to 16 hours. Results provide evidence for amplification method-dependent biases in gene expression data.
GPL8300:
[HG_U95Av2] Affymetrix Human Genome U95 Version 2 Array
Citation:
Ma C, Lyons-Weiler M, Liang W, LaFramboise W et al. In vitro transcription amplification and labeling methods contribute to the variability of gene expression profiling with DNA microarrays. J Mol Diagn 2006 May;8(2):183-92. PMID: 16645204
