Yersinia pestis AS 2509 - SRA

SRX286305: Yersinia pestis AS 2509
1 ILLUMINA (Illumina Genome Analyzer IIx) run: 5.7M spots, 471.4M bases, 242.7Mb downloads

Design: The DNA samples were prepared for multiplexed (single-read, 82 cycles) sequencing using Illumina GAIIx genome analyzer (Illumina Inc., San Diego, CA). For each isolate, genomic library preps were generated using Nextera DNA Sample Prep Kit. Post library quality control and quantification was done using BioAnalyzer 2100 high-sensitivity chips and KAPA SYBR FAST Universal 2X qPCR Master Mix. Post processing of reads was performed by the RTA/SCS v1.9.35.0 and CASAVA 1.8.0. Reads were trimmed back to the Q30 level using CLCBio's quality_trim program, CutAdapt v0.95 was used to excise adapter and transposon contamination.

Submitted by: THE GEORGE WASHINGTON UNIVERSITY

Study: Yersinia pestis Genome sequencing

PRJNA194125 • SRP022877 • All experiments • All runs

show Abstracthide Abstract

Illumina sequencing of barcoded sample library of Yersinia pestis for the study “Investigating the diversity of Yersinia pestis isolates."

Sample: Generic sample from Yersinia pestis

SAMN01991276 • SRS429128 • All experiments • All runs

Organism: Yersinia pestis AS 2509

Library:

Name: Yersinia

Instrument: Illumina Genome Analyzer IIx

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: SINGLE

Spot descriptor:

1 forward

Runs: 1 run, 5.7M spots, 471.4M bases, 242.7Mb

Run	# of Spots	# of Bases	Size	Published
SRR867064	5,748,284	471.4M	242.7Mb	2013-05-23

ID:: 404195

SRA

Sequence Read Archive

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