AHE of Aleucosia directa - SRA

SRX7428582: AHE of Aleucosia directa
1 ILLUMINA (NextSeq 500) run: 4M spots, 1.2G bases, 532.1Mb downloads

Design: The DNA was fragmented by sonication with a Bioruptor Pico (Diagenode, USA), using 50 L of the DNA extractions in 0.1 ml tubes (Cat. No. C30010015), targeting a modal fragment size of 350 base pairs. Libraries were built using the NEBNext Ultra II DNA Library Prep Kit (NEB #E7645L) with NEBNext Multiplex Oligos for Illumina (96 Index Primers) (NEB #E6609L), with two-sided size selection around a mode of 480 base pairs. Target enrichment through hybridization followed the Agilent SureSelectXT Target Enrichment Protocol (Version C2), with a modification at the end whereby the clean, target-enriched DNA suspension was incubated at 95 C for 5 minutes, then immediately put onto the magnetic separator to take off the enriched supernatant. The probe set used the published AHE Diptera Probe set and targeted 559 loci. Enriched libraries were amplified using Phusion Hot Start II High-Fidelity DNA Polymerase (Thermo Fisher Scientific, F549L). PCR cycling consisted of an initial denaturing step at 98 C for 30 s, followed by 8 cycles of denaturing at 98 C for 10 s, annealing at 60 C for 30 s, elongation at 72 C for 30 s, and a final elongation step at 72 C for 10 min.

Submitted by: Commonwealth Scientific and Industrial Research Organization

Study: Phylogenomics of bee flies (Diptera, Bombyliidae)

PRJNA596828 • SRP238502 • All experiments • All runs

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This project is aiming to reconstruct the phylogeny and divergence times of bee flies (Diptera: Bombyliidae) using anchored hybrid enrichment and transcriptomics data. The Bombyliidae (bee flies) is one of the most species-rich families in lower Brachycera (Diptera), comprising 15 subfamilies, over 250 genera and over 5,000 described species. Adult bee flies feed on nectar and pollen and are important pollinators, and their larvae are almost exclusively parasitoids, feeding on the immatures of a wide range of insect hosts.

Sample: Invertebrate sample from Aleucosia directa

SAMN13639819 • SRS5874488 • All experiments • All runs

Organism: Aleucosia directa

Library:

Name: XLI_38_S34

Instrument: NextSeq 500

Strategy: Targeted-Capture

Source: GENOMIC

Selection: Hybrid Selection

Layout: PAIRED

Runs: 1 run, 4M spots, 1.2G bases, 532.1Mb

Run	# of Spots	# of Bases	Size	Published
SRR10753991	3,981,023	1.2G	532.1Mb	2020-11-24

ID:: 9720450

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