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SRX8785782: MiSeq of Cladocopium ITS2 type C15b.2:Goniastrea retiformis coral host
1 ILLUMINA (Illumina MiSeq) run: 1 spots, 296 bases, 77,780b downloads

Design: A 350bp segment of the internal transcribed spacer region 2 (ITS2) rDNA region was amplified using Symbiodiniaceae specific primers, ITS-Dino-forward and its2rev2-reverse. Each primer also contained a universal linker, for downstream incorporation of Illumina adapters and barcodes. Following barcoding PCR, samples were pooled and sequenced on an Illumina MiSeq (250bp paired-end Reagent Nano Kit v2).
Submitted by: Old Dominion University
Study: Symbiodiniaceae diversity in massive corals from backreef lagoons in American Samoa
show Abstracthide Abstract
Investigation of community structure and photophysiology of coral-algal endosymbionts (Family Symbiodiniaceae) following transplantation into a highly variable thermal reef environment. Two coral host species (<i>Porites lobata</i> and <i>Goniastrea retiformis</i>) were transplanted from two nearby backreef lagoons into the highly variable lagoon for one year, and at six and twelve months, acute heat stress experiments were conducted to elucidate thermal tolerances of the coral host and algal symbiont. DNA was extracted and symbiont specific ITS2 amplicons were amplified prior to high-throughput sequencing on Illumina's MiSeq platform.
Sample: Cladocopium sp.
SAMN15585744 • SRS7054413 • All experiments • All runs
Library:
Name: Cladocopium ITS2 type C15b.2
Instrument: Illumina MiSeq
Strategy: AMPLICON
Source: GENOMIC
Selection: PCR
Layout: SINGLE
Runs: 1 run, 1 spots, 296 bases, 77,780b
Run# of Spots# of BasesSizePublished
SRR12282358129677,780b2020-07-21

ID:
11421584

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