RNA-Seq of total RNA from virus infected passion fruit: leaf sample - SRA

SRX5232034: RNA-Seq of total RNA from virus infected passion fruit: leaf sample
1 ILLUMINA (Illumina MiSeq) run: 4.2M spots, 1.3G bases, 631.9Mb downloads

Design: Poly A containing mRNA molecules from diluted total RNA (1g) were purified using poly-T oligo attached magnetic beads. The purified poly-A RNA was then fragmented and primed for 1st and 2nd strand cDNA synthesis. After cDNA sythensis, the 3' ends were adenylated by addition of a single 'A' nucleotide to the 3' ends. Indexing adapters were then ligated to the ends of the ds cDNA followed by enrichment of DNA fragments by PCR with adapter molecules on both ends. This was followed by checking the quality (validation) of enriched DNA templates by loading 1l of re-suspended construct on a 2100 Bioanalyzer using an Agilent DNA 1000 chip. A final product with a band of 260bp indicated the right size and purity of the sample. After library validation, the indexed DNA library was then normalized to 10nM using Tris-HCl 10 mM, pH8.5 with 0.1% Tween 20 and sequenced.

Submitted by: National Semi-Arid Resources Research Institute, National Agricultural Research Organization

Study: Discovery of passion fruit viruses in Uganda by deep sequencing

PRJNA513986 • SRP178064 • All experiments • All runs

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The aims of this study were to detect and identify viruses affecting passion fruit production in the main growing areas of Uganda; characterize the level of molecular diversity of passion fruit viruses ; and develop specific molecular diagnostic tools for major passion fruit viruses in Uganda

Sample:

SAMN10717829 • SRS4235626 • All experiments • All runs

Organism: Ugandan passiflora virus

Library:

Name: S8_S5_L001_R1

Instrument: Illumina MiSeq

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 4.2M spots, 1.3G bases, 631.9Mb

Run	# of Spots	# of Bases	Size	Published
SRR8423040	4,216,302	1.3G	631.9Mb	2019-01-09

ID:: 7068100

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