transcriptomic analysis of XX fugu gonad under MT treatment - SRA

Links from BioProject

SRX8862687: transcriptomic analysis of XX fugu gonad under MT treatment
1 ILLUMINA (Illumina HiSeq 2500) run: 23.3M spots, 7G bases, 2.1Gb downloads

Design: mRNA was isolated by NEBNext Poly (A) mRNA Magnetic Isolation Module (New England Biolabs Inc., Ipswich, MA, USA). The cDNA library was constructed following the manufacturers instructions of NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs Inc., Ipswich, MA, USA 0) and NEBNext Multiplex Oligos for Illumina (New England Biolabs Inc., Ipswich, MA, USA). In briefly, the enriched mRNA was fragmented into approximately 202nt RNA inserts, which were used to synthesize the first-strand cDNA and the second cDNA. The double-stranded cDNA was performed end-repair/dA-tail and adaptor ligation. The suitable fragments were isolated by Agencourt AMPure XP beads (Beckman Coulter, Inc. Beverly, MA, USA), and enriched by PCR amplification.

Submitted by: Dalian Ocean University

Study: Takifugu rubripes Raw sequence reads mF

PRJNA647642 • SRP274692 • All experiments • All runs

show Abstracthide Abstract

transcriptomic analysis of fugu XX gonad under MT treatment

Sample:

SAMN15690921 • SRS7124408 • All experiments • All runs

Organism: Takifugu rubripes

Library:

Name: MTF

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 23.3M spots, 7G bases, 2.1Gb

Run	# of Spots	# of Bases	Size	Published
SRR12363453	23,279,377	7G	2.1Gb	2021-02-14

ID:: 11521432

SRA

Sequence Read Archive

Result Filters

Send to:

Links from BioProject

Supplemental Content

Related information

Recent activity