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SRX7258917: Targeted amplicon deep sequencing of 18 rRNA genes nUPDx (nUPDx method validation)
1 ILLUMINA (Illumina MiSeq) run: 10,500 spots, 4.9M bases, 2.2Mb downloads

Design: Amplicon enrichment NEBNext UltraII DNA Library Prep Kit
Submitted by: CDC
Study: Universal Parasite Detection
show Abstracthide Abstract
Targeted amplicon deep sequencing (TADS) of the 16S rRNA gene is commonly used to explore and characterize the bacterial microbiome. Meanwhile, attempts to apply TADS to the detection and characterization of entire parasitic communities have been hampered since conserved regions of their rRNA genes are also conserved in their eukaryotic hosts. As a result, targeted amplification of rRNA from clinical samples using universal primers frequently results in competitive priming and preferential amplification of host DNA. The Universal Parasite Detection project aims to address this by developing new approaches that result in preferential amplification of parasite-derived DNA amongst a larger background of host DNA prior to the application of TADS.
Sample: 18-Ll-D2-S12-Rep2
SAMN13412554 • SRS5754960 • All experiments • All runs
Organism: Loa loa
Library:
Name: 18-Ll-D2_S12
Instrument: Illumina MiSeq
Strategy: AMPLICON
Source: GENOMIC
Selection: PCR
Layout: PAIRED
Runs: 1 run, 10,500 spots, 4.9M bases, 2.2Mb
Run# of Spots# of BasesSizePublished
SRR1057842910,5004.9M2.2Mb2019-12-04

ID:
9530982

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