Pacbio sequencing of Candida glabrata: BG3995 - SRA

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SRX8337409: Pacbio sequencing of Candida glabrata: BG3995
1 PACBIO_SMRT (PacBio RS II) run: 49,564 spots, 474.8M bases, 308.7Mb downloads

Design: Genomic DNA of strain BG3995 was made by preparation of spheroplasts, followed by lysis and spooling of high molecular weight genomic DNA after ethanol precipitation. The genomic DNA was sheared to 30 kb with MegaRupter. The sheared DNA was diluted to under 50 ng/l with TE, purified using a 1X AMPure bead cleanup, and elutied in 62 l of EB. The library was prepared using the standard PacBio 30kb protocol. Briefly, after fragmentation, the fragmented DNA is end repaired and A-tailed followed by SMRTbell hairpin ligation. Upon completion of the library, the sample was then size selected on the Blue Pippin to the range of 20-50kb. The size selected library was then sequenced on PacBio RS II using PacBio P6/C4 chemistry

Submitted by: Johns Hopkins Medical Institution

Study: [Candida] glabrata Genome sequencing and assembly of strain BG3995

PRJNA630578 • SRP261450 • All experiments • All runs

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De novo genome assembly of Candida glabrata strain BG3995.

Sample: Pathogen: clinical or host-associated sample from [Candida] glabrata, strain BG3995

SAMN14840177 • SRS6655974 • All experiments • All runs

Organism: Nakaseomyces glabratus

Library:

Name: 583.filtered_subreads

Instrument: PacBio RS II

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: SINGLE

Runs: 1 run, 49,564 spots, 474.8M bases, 308.7Mb

Run	# of Spots	# of Bases	Size	Published
SRR11784994	49,564	474.8M	308.7Mb	2021-08-31

ID:: 10845518

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Sequence Read Archive

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