GBMF Marine Phage Sequencing Project - SRA

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SRX042508: GBMF Marine Phage Sequencing Project
2 LS454 (454 GS FLX Titanium) runs: 177,647 spots, 80.4M bases, 182.4Mb downloads

UUID: 1ea4f003-2caf-44c7-ae1b-c3699123ed03

Design: International collaboration to sequence, assemble, and annotate approximately 200 diverse marine phage/virus genomes and to sequence approximately 50 viral metagenomes from an array of marine environments. DNA sequencing using 454 technology.

Submitted by: Broad Institute (BI)

Study: 4 samples from EPR hydrothermal vent uncultured phage metagenome

PRJNA47485 • SRP005751 • All experiments • All runs

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Sample 4472 Induced RNA isolated from diffuse flow hydrothermal vent seawater; sample description: placed near shimmering water, near Marker 28 and a Riftia patch. With lots of biology, near a prototrap 109. Bio-9/P-vent Sample 4472 Induced ssDNA isolated from diffuse flow hydrothermal vent seawater; sample description: placed near shimmering water, near Marker 28 and a Riftia patch. With lots of biology, near a prototrap 109. Bio-9/P-vent Sample 4473 Free RNA isolated from diffuse flow hydrothermal vent seawater; sample description: placed near crabs, next to shimmering water (370 C) black smoker, placed on top of tevnia patch behind shimmering water. Extra ~50 L of water, expanding bags. Marker 28 (Trick or Treat area) Sample 4473 Free ssDNA isolated from diffuse flow hydrothermal vent seawater; sample description: placed near crabs, next to shimmering water (370 C) black smoker, placed on top of tevnia patch behind shimmering water. Extra ~50 L of water, expanding bags. Marker 28 (Trick or Treat area)

Sample: Generic sample from hydrothermal vent metagenome

SAMN00215761 • SRS172692 • All experiments • All runs

Organism: hydrothermal vent metagenome

Library:

Name: 454-4149

Instrument: 454 GS FLX Titanium

Strategy: WGS

Source: METAGENOMIC

Selection: PCR

Layout: SINGLE

Spot descriptor:

barcode

Group tag	Basecalls
Kinshasa	TGACGAC

12 forward

Experiment attributes: (show all 6 attributes...) (hide...)

analysis_type: DNA Viral 454 Alignment and Assembly

gssr_id: 46630.1

lsid: BROAD:SEQUENCING_SAMPLE:46630.1

material_type: Genomic DNA

project: G3262

work_request: 21182

Pipeline: show...hide...

Name	Step	Program	Version
base caller	2010-04-17 19:17:48.0	454BaseCaller	2.3

Runs: 2 runs, 177,647 spots, 80.4M bases, 182.4Mb

Run	# of Spots	# of Bases	Size	Published
SRR100523	174,663	79.3M	179.6Mb	2011-06-30
SRR107220	2,984	1.1M	2.8Mb	2011-06-30

ID:: 50780

SRA

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