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SRX14737131: ddRADSeq of Chorthippus saulcyi moralesi: adult female muscle
1 ILLUMINA (Illumina HiSeq 2500) run: 2.3M spots, 333.7M bases, 197.6Mb downloads

Design: We used NucleoSpin Tissue kits (Macherey-Nagel, Duren, Germany) to extract and purify genomic DNA from the hind femur of each individual. We processed genomic DNA into different genomic libraries (48 individuals/library) using the double-digestion restriction-site associated DNA sequencing procedure (ddRAD-seq) described in Peterson et al. (2012) (PLOS One, 7: e37135). In brief, we digested DNA with the restriction enzymes MseI and EcoRI (New England Biolabs, Ipswich, MA, USA) and ligated Illumina adaptors including unique 7-bp barcodes to the digested fragments of each individual. We pooled ligation products and size-selected them between 475-580 bp with a Pippin Prep machine (Sage Science, Beverly, MA, USA). We amplified the fragments by PCR with 12 cycles using the iProofTM High-Fidelity DNA Polymerase (BIO-RAD, Hercules, CA, USA) and sequenced the libraries in single-read 150-bp lanes on an Illumina HiSeq2500 platform at The Centre for Applied Genomics (SickKids, Toronto, ON, Canada).
Submitted by: Estacion Biologica de Donana (EBD-CSIC)
Study: RADseq for Chorthippus subgenus Glyptobothrus (Orthoptera: Acrididae)
show Abstracthide Abstract
The goal of this project is to integrate next generation sequencing (NGS) data, detailed phenotypic information, and spatial modelling to infer the processes shaping patterns of genetic, ecological and phenotypic divergence at different evolutionary scales. This project uses as model system a recent evolutionary radiation of montane grasshoppers (genus Chorthippus, subgenus Glyptobothrus) to understand the consequences of past climatic changes and the role of geography, environment and adaptation processes on determining 1) species diversification and 2) regional and local intraspecific patterns of genomic and phenotypic variation. Thus, this project aims to track the diversification process from those stages that shape early genetic and phenotypic divergence at small spatiotemporal scales through the speciation end, both of which are well represented in this species complex composed by several recently diverged taxa with contrasting ecological and habitat requirements.
Sample:
SAMN27281807 • SRS12499028 • All experiments • All runs
Library:
Name: CMorChia01
Instrument: Illumina HiSeq 2500
Strategy: RAD-Seq
Source: GENOMIC
Selection: Restriction Digest
Layout: SINGLE
Runs: 1 run, 2.3M spots, 333.7M bases, 197.6Mb
Run# of Spots# of BasesSizePublished
SRR186101602,317,095333.7M197.6Mb2022-04-08

ID:
21093016

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