Low-coverage whole genome sequencing of Aldabrachelys gigantea: indiv... - SRA

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SRX14775739: Low-coverage whole genome sequencing of Aldabrachelys gigantea: individual MLR3
1 ILLUMINA (Illumina NovaSeq 6000) run: 20.5M spots, 6.2G bases, 1.9Gb downloads

Design: More than 200 ng of DNA per sample was sent to Novogene Company (Cambridge, United Kingdom) for library preparation and sequencing. Briefly, the genomic DNA was randomly fragmented to the size of 350 bp, and then DNA fragments were end-polished, A-tailed, ligated with the adapters of Illumina sequencing, and PCR enriched with primers of P5 and P7 oligos. The PCR products as the final construct of the libraries were purified (AMPure XP system), and this was followed by a quality control test that included size distribution by Agilent 2100 Bioanalyzer (Agilent Technologies, CA, USA), and molarity measurement using real-time PCR. The quality-checked libraries were then sequenced on the Illumina Novaseq 6000 platform with paired-end runs of 150 bp read length.

Submitted by: University of Zurich

Study: Aldabrachelys gigantea genome sequencing and assembly

PRJNA822095 • SRP367997 • All experiments • All runs

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Aldabrachelys gigantea (Aldabra giant tortoise) genome assembly

Sample:

SAMN27403219 • SRS12535314 • All experiments • All runs

Organism: Aldabrachelys gigantea

Library:

Name: FDSW210068044-1r

Instrument: Illumina NovaSeq 6000

Strategy: WGS

Source: GENOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 20.5M spots, 6.2G bases, 1.9Gb

Run	# of Spots	# of Bases	Size	Published
SRR18674088	20,520,692	6.2G	1.9Gb	2022-08-07

ID:: 21150987

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