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SRX20664278: Amplicon sequencing of Human immunodeficiency virus 1:Amp_LR3_S+b_2
1 ILLUMINA (Illumina NovaSeq 6000) run: 17.2M spots, 3.7G bases, 1Gb downloads

Design: I added a UMI to each viral mRNA molecule immediately downstream the barcode sequence by reverse transcription. Then I amplifed the barcode region using a set of primers annealed to the upstream of the barcode and the reverse transcription primer. The PCR product was prepared for sequencing using NEBNext Library Preparation Module.
Submitted by: (UCLA)
Study: Human immunodeficiency virus 1 Raw sequence reads
PRJNA970831 • SRP438275 • All experiments • All runs
show Abstracthide Abstract
Integration site sequencing of HIV-1 and SHIV-1
Sample: Amp_LR3_S+b_2
SAMN35719167 • SRS17960489 • All experiments • All runs
Organism: Human immunodeficiency virus 1
Library:
Name: Amp_LR3_S+b_2
Instrument: Illumina NovaSeq 6000
Strategy: AMPLICON
Source: VIRAL RNA
Selection: PCR
Layout: PAIRED
Runs: 1 run, 17.2M spots, 3.7G bases, 1Gb
Run# of Spots# of BasesSizePublished
SRR2490236917,175,0913.7G1Gb2024-06-04

ID:
28093651

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