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SRX22367149: Transcriptome of Diceraeus furcatus nymphs
1 ILLUMINA (Illumina NovaSeq 6000) run: 49.2M spots, 14.8G bases, 4.6Gb downloads

Design: Total RNA was extracted from whole bodies of ten first instar nymphs of each species using TRIzol Reagent (Invitrogen, Carlsbad, CA, USA), following manufacturers instructions. Total RNA was quantitated on a Nanodrop 2000c spectrophotometer (Thermo Scientific, Wilmington, USA) and its quality assessed with 1% (w/v) agarose gel electrophoresis. Immediately after extraction, 2 g of total RNA of each sample was lyophilized and shipped to Novogene (Davis, USA) for sequencing on an Illumina NovaSeq platform. The construction of cDNA libraries was performed from RNA samples for Illumina paired end (PE) sequencing following the Illumina protocol. The sequencing strategy was PE150, which gives 150bp read from each end to make a 300bp sequenced fragment.
Submitted by: Universidad Nacional de La Plata
Study: De novo assembly and comparative transcriptomics of the soybean stink bug complex members Edessa meditabunda, Piezodorus guildinii, and Diceraeus furcatus
show Abstracthide Abstract
The stink bug complex (Hemiptera: Pentatomidae) includes insects known by its tendency to release a bad odor when disturbed or when crushed. Stink bugs are phytophagous insects considered major pests of crops worldwide, especially in the Neotropical Region that concentrates the largest total production of basic agricultural commodities such as soybean, maize, cotton, rice, and wheat (Panizzi et al., 2022). Argentina is the fourth largest world soybean producer with 13.809.328 ha cultivated in 2021, representing about 12.5% of worldwide soybean cultivated area (source: http://www.fao.org/faostat/en/#data). The more frequent species within the stink bug complex found in the Argentinean core area of soybean production (provinces of Buenos Aires, Santa Fe, and Cordoba) are the southern green stink bug Nezara viridula (Linnaeus), the brown-winged stink bug Edessa meditabunda (Fabricius), the redbanded stink bug Piezodorus guildinii (Westwood), and the green-belly stink bug Diceraeus furcatus (F.) (Sosa and Gamundi, 2008; Jacobi et al., 2022; Balbi et al., 2023).
Sample: Diceraeus furcatus transcriptome
SAMN38058404 • SRS19415048 • All experiments • All runs
Library:
Name: D. furcatus nymphs
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Runs: 1 run, 49.2M spots, 14.8G bases, 4.6Gb
Run# of Spots# of BasesSizePublished
SRR2666710149,215,38514.8G4.6Gb2024-06-19

ID:
30346543

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