SRX22583089: Low-P-25C_1
1 DNBSEQ (DNBSEQ-T7) run: 21.6M spots, 6.5G bases, 3.7Gb downloads
Design: 1)mRNA enrichment: Oligo dT Selection or rRNA depletion; 2)RNA fragment and reverse transcription: Fragment the RNA and reverse transcription to double-strand cDNA (dscDNA) by N6 random primer; 3)End repair, add A tailing and adaptor ligation:The synthesized cDNA was subjected to end-repair and then was 3 adenylated. Adaptors were ligated to the ends of these 3 adenylated cDNA fragments; 4)PCR amplification:The ligation products were purified and many rounds of PCR amplification were performed to enrich the purified cDNA template using PCR primer; 5)Denature and cyclization:Denature the PCR product by heat and the single strand DNA is cyclized by splint oligo and DNA ligase; 6) Sequencing on DNBSEQ platform.
Submitted by: Xiamen University
Study:
Transcriptomic sequencing data of Symbiodiniaceae Clade A Symbiodinium sp. under phosphorus-limitation and heat stressLibrary:
Name: LP25_1
Instrument: DNBSEQ-T7
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: Oligo-dT
Layout: PAIRED
Runs:
1 run, 21.6M spots, 6.5G bases, 3.7Gb