SRX23311498: RNA-seq of m474b
1 ILLUMINA (NextSeq 2000) run: 9.2M spots, 754.6M bases, 263.1Mb downloads
Design: For RNA-seq, ribosomal RNA was removed using the QIAseq FastSelect -rRNA HMR Kit (Qiagen) and libraries were prepared using the NEBNext Ultra II Directional Library Prep kit (New England Biolabs). The protocol for the library preparation kit was followed, with the modifications suggested by the rRNA removal reagent protocol. Finished libraries were analyzed on the TapeStation 2200 (Agilent) and sequenced using a 1 x 120 single-end configuration on the Illumina NextSeq 2000 (Illumina)..
Submitted by: Utah State University
Study:
Candid#1 variant reversion in huTfR1 miceshow Abstracthide AbstractThe goal of the study was to assess additional reversion events in Candid#1 427F or 168T single revertants during infection in huTfR1 mice.
Library:
Name: m474b
Instrument: NextSeq 2000
Strategy: RNA-Seq
Source: VIRAL RNA
Selection: RT-PCR
Layout: SINGLE
Runs:
1 run, 9.2M spots, 754.6M bases, 263.1Mb