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SRX23653431: RNA-seq of Laminaria hyperborea
1 ILLUMINA (Illumina HiSeq 4000) run: 51.1M spots, 15.4G bases, 4.7Gb downloads

Design: Total RNA extraction was conducted following the method described by Heinrich et al., (2012). RNA quality (OD260/280 ration values) and concentration (ng/l) were obtained using a DeNovix DS-11 Series Spectrophotometer/Fluorometer (22.96 ng/l for Chondrus crispus; 235.47 ng/l for Laminaria hyperborean; 94.697 ng/l for Laminaria ochroleuca). One sample of total RNA of each target species was used for cDNA libraries preparation, using a TruSeq Stranded Total RNA Library protocol with RiboZero Plant, and short reads paired-end sequences were generated in an Illumina HiSeq4000 platform, at Macrogen, Inc.
Submitted by: Interdisciplinary Centre of Marine and Environmental Research (CIIMAR) / University of Porto
Study: Raw sequence reads used for transcriptome assembly of three intertidal macroalgae species: Chondrus crispus, Laminaria hyperborea, and Laminaria ochroleuca.
show Abstracthide Abstract
While genomic tools hold significant promise for applications in ecosystem conservation and management, their current utilization remains limited. Intertidal macroalgae species experience continuous exposure to diverse stressors, demonstrating a heightened potential for adaptation and acclimation. Many of these species act as foundation species, playing a pivotal ecological role in ecosystems. For this reason, it is crucial to comprehend the adaptive capacities of these species to allow an effective management and conservation of communities. In this study, we provide the transcriptomes of three intertidal macroalgae species: Chondrus crispus, Laminaria hyperborea, and Laminaria ochroleuca. The resulting assemblies yield N50 values within the range of 619-578 bp, with BUSCO scores ranging from 56.1% to 27.5%. These assemblies have undergone both structural and functional annotation were made available.
Sample: Sample from Laminaria hyperborea
SAMN39979020 • SRS20486295 • All experiments • All runs
Library:
Name: SSH14LH11
Instrument: Illumina HiSeq 4000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: RANDOM
Layout: PAIRED
Runs: 1 run, 51.1M spots, 15.4G bases, 4.7Gb
Run# of Spots# of BasesSizePublished
SRR2800087051,065,13915.4G4.7Gb2024-02-18

ID:
31935665

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