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SRX23826855: Metagenomics of Hymeniacidon heliophila host microorganisms
1 ILLUMINA (Illumina MiSeq) run: 85,895 spots, 48.3M bases, 29.6Mb downloads

Design: The primer pair 515F-806R was used to amplify the V4-region of the 16S rRNA gene of Bacteria and Archaea using the extracted DNA (Apprill et al., 2015; Parada et al., 2016) and aliquots were sequenced at the NGS Solues Genmicas (Piracicaba, SP. Brazil). The reaction mixture (25 L) was prepared with 2.5 L of template DNA (20 ng/L), 0.20 nM of both primers and 2X PCRBio Ultra Mix (PCRBiosystems). The thermal cycle was set as following: 3 min at 95C, 30 cycles of 30 s at 95C, 30 s at 60C and 30 s at 72C. Final extension was performed for 10 min at 72C (Hardoim et al., 2021). The amplicons obtained were subjected to Illumina sequencing using MiSeq platform
Submitted by: University of Sao Paulo
Study: Effects of Di(2ethylhexyl) phthalate (DEHP) in the physiology of Hymeniacidon heliophila
show Abstracthide Abstract
Research on marine pollution and its impacts on biota has focused over the last 15years on an important anthropic pollutant, plastic, and specifically on the effects ofmicroplastics on marine organisms. Of varied types and size, they have different originsdepending on their final use and carry chemical pollutants that can contribute todiversification of damage in organisms exposed to these microparticles. Although wehave an extensive literature on the physical effects of microplastics on marine biota, thechemical effects of additives and pollutants adsorbed to its structure are still poorlyknown, especially in basal metazoan organisms such as sponges. Among additives withpresence in the marine ecosystem, DEHP (Di(2ethylhexyl phthalate)), a product used asplasticizer to increase the malleability of polymers such as PVC, has the greaterconcentration. Potent endocrine disruptor in vertebrates, recently DEHP was found insponges tissues, but there are no reports about its possible chemical effects on thephysiology of these filter-feeder organisms. This work seeks to obtain, with the use ofclassical methodologies, such as histology, and modern ones, such as metagenomics, thefirst reports of the effects of exposure to DEHP in the sponge Hymeniacidon Heliophila,at different biological levels.
Sample:
SAMN40251110 • SRS20648933 • All experiments • All runs
Library:
Name: Hh09F2
Instrument: Illumina MiSeq
Strategy: AMPLICON
Source: METAGENOMIC
Selection: PCR
Layout: PAIRED
Runs: 1 run, 85,895 spots, 48.3M bases, 29.6Mb
Run# of Spots# of BasesSizePublished
SRR2821459985,89548.3M29.6Mb2024-03-04

ID:
32128565

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