Metagenomic sequencing of Kebrit Deep Brine Pool: Red Sea - SRA

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SRX24228668: Metagenomic sequencing of Kebrit Deep Brine Pool: Red Sea
1 ILLUMINA (Illumina HiSeq 4000) run: 34.6M spots, 10.4G bases, 4.3Gb downloads

Design: The metagenomic libraries were performed using the Ovation Ultralow V2 DNA-Seq Library preparation kit (NuGen) following the manufacturers instructions. Briefly, a range of 50 to 100 ng of DNA from each fraction was diluted in 120 L of ultra-pure water (Ambion) in a Covaris snap cap microtube (PN 520045, Covaris). The DNA fragmentation was performed by Covaris M220 sonication to acquire 300 bp DNA fragments using a modified sonication protocol: 70 seconds treatment time, 200 cycles per bust, 20% duty factor, 50 W peak incident power. The fragmented DNA was end-repaired, tagged with a univocal index for each sample, randomly amplified, and cleaned with AMPure XP Beads (Beckman Coulter) following the NuGen library preparation protocol. The quality of the libraries was assessed by Bioanalyzer 2100 (Agilent). The DNA was quantified with a Qubit 3.0 dsDNA high-sensitivity quantification assay kit, and the libraries were pooled in a final 20 nM pool. The pool quality was assessed again by Bioanalyzer 2100 (Agilent) and qPCR with Kapa Library Quantification kit (KAPA Biosystems). The sequencing was performed at the Biological Core Lab (BCL) at King Abdullah University for Science and Technology (KAUST) with Illumina Hiseq 4000 sequencing platform (2 x 150 bp cycle).

Submitted by: King Abdullah University of Science and Technology

Study: Metagenomics of Kebrit brine pools

PRJNA1096827 • SRP501261 • All experiments • All runs

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Metagenomic sequencing to understand the microbiome of the Kebrit brine pool, Red Sea

Sample:

SAMN40763605 • SRS20998204 • All experiments • All runs

Organism: seawater metagenome

Library:

Name: M_18_3280_K6_B01

Instrument: Illumina HiSeq 4000

Strategy: AMPLICON

Source: METAGENOMIC

Selection: size fractionation

Layout: PAIRED

Runs: 1 run, 34.6M spots, 10.4G bases, 4.3Gb

Run	# of Spots	# of Bases	Size	Published
SRR28630809	34,572,936	10.4G	4.3Gb	2024-06-30

ID:: 32540690

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