WGS of Eulemur rufifrons - SRA

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SRX24340742: WGS of Eulemur rufifrons
1 OXFORD_NANOPORE (MinION) run: 36.6M spots, 58.8G bases, 48.5Gb downloads

Design: DNA was isolated from the skin sample with Monarch High Molecular Weight (HMW) Extraction Kit for Tissue (New England Biolabs) and from the spleen sample withDNeasy Blood and Tissue kit (Qiagen). Two library preps were performed with Field Sequencing Kit (Oxford Nanopore SQK-LRK001) using HMW DNA and five library preps were performed with Ligation Sequencing kit (Oxford Nanopore SQK-LSK109) using both HMW and non-HMW DNA. Ligation Sequencing kit prepared libraries were eluted in up to 48 microliters and separated into 12 microliter aliquots. All libraries were sequenced for up to 72 hours on a total of ten R9.4.1 minION flow cells. The FAST5 files produced across all sequencing efforts were re-basecalled using Dorado (v. 0.3.4, model r941_min_sup_g507).

Submitted by: Rutgers, The State University of New Jersey

Study: Whole genome sequencing of Eulemur rufifrons

PRJNA1103329 • SRP503439 • All experiments • All runs

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The red-fronted brown lemur (Eulemur rufifrons) is an endangered primate endemic to Madagascar (IUCN Red List). This project conducted whole genome sequencing of Eulemur rufifrons for reference genome assembly.

Sample:

SAMN41050454 • SRS21100893 • All experiments • All runs

Organism: Eulemur rufifrons

Library:

Name: eul_ruf

Instrument: MinION

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: SINGLE

Runs: 1 run, 36.6M spots, 58.8G bases, 48.5Gb

Run	# of Spots	# of Bases	Size	Published
SRR28775784	36,596,792	58.8G	48.5Gb	2024-04-23

ID:: 32654813

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