Illumina MiSeq paired end sequencing ofSAMD00048496:ST5e3_3 - SRA

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DRX051556: Illumina MiSeq paired end sequencing ofSAMD00048496:ST5e3_3
1 ILLUMINA (Illumina MiSeq) run: 15,000 spots, 6.3M bases, 3.9Mb downloads

Submitted by: Japan Agency for Marine-Earth Science and Technology (JAMSTEC)

Study: Quantitative Sequencing by using random-sequence barcodes

PRJDB4568 • DRP003217 • All experiments • All runs

show Abstracthide Abstract

A novel method for quantitative sequencing enabling absolute quantification of template DNA molecule with sequence information was developed. For testing the concept of method, environmental samples from paddy field soil and beach sand were used.In addition, we validated the method with mock microbial communities which consist gDNA of Methanocaldococcus jannaschii, Halomonas elongata, Sulfolobus tokodaii, Bacillus subtilis subsp. subtilis, Streptomyces avermitilis, and Paracoccus denitrificans.

Sample: 16S rRNA gene of S.tokodaii ID ST5e3_3

SAMD00048496 • DRS033201 • All experiments • All runs

Organism: Sulfurisphaera tokodaii

Library:

Name: ST5e3_3

Instrument: Illumina MiSeq

Strategy: AMPLICON

Source: GENOMIC

Selection: PCR

Layout: PAIRED

Construction protocol: Random-tag qantitative sequence (Ratq-seq) was applied. Primer sequences were not removed. Eight bases at both ends are random-tag (N8).

Spot descriptor:

1 forward154 reverse

Runs: 1 run, 15,000 spots, 6.3M bases, 3.9Mb

Run	# of Spots	# of Bases	Size	Published
DRR056920	15,000	6.3M	3.9Mb	2016-08-02

ID:: 2869086

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