RNA-seq of Limulus polyphemus: central nervous system - SRA

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SRX2153488: RNA-seq of Limulus polyphemus: central nervous system
1 ILLUMINA (Illumina HiSeq 1000) run: 78.3M spots, 15.7G bases, 10.2Gb downloads

Design: Animal was sacrificed at 08:00 and it's entire central nervous system (protocerebrum, subesophageal ganglia and ventral nerve cord) was removed and frozen on dry ice. The RNA was extracted using Ambion TRIzol and purified using Qiagen's Rneasy Mini kit. Libraries were prepared using Illumina's TruSeq RNA Sample Prep LT and loaded on the sequencer at ~12pM.

Submitted by: University of New Hampshire

Study: Limulus polyphemus Draft Transcriptome and Raw Sequence Reads

PRJNA340463 • SRP087725 • All experiments • All runs

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In this study we developed a draft transcriptome for Limulus polyphemus and then compared the genes expressed during high and low tides and during the day vs the night. Particular attention was paid to putative core and accessory circadian genes. We identified these, and then compared their expression, using RPKM values, across the different conditions (tides and Light:Dark(L:D)).

Sample:

SAMN05713726 • SRS1683997 • All experiments • All runs

Organism: Limulus polyphemus

Library:

Name: DHT

Instrument: Illumina HiSeq 1000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 78.3M spots, 15.7G bases, 10.2Gb

Run	# of Spots	# of Bases	Size	Published
SRR4215561	78,262,857	15.7G	10.2Gb	2016-09-14

ID:: 3148268

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