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SRX2690179: DNA seq of human: Adult female melanoma: Sample N46
1 ILLUMINA (Illumina HiSeq 2000) run: 60.2M spots, 9.2G bases, 4.3Gb downloads

Design: The initial genomic DNA was sheared using low Tris-EDTA buffer. KAPA Hyper Prep Kit (#KK8504) was used for end repair, A-base addition, adapter ligation, and library enrichment PCR. The Agilent SureSelect-XT Target Enrichment was used for exon target capture. The libraries were then normalized to equal concentrations and were quantified using the KAPA Library Quantification Kit.
Submitted by: NCI-phs001257
Study: Loss of IFN-g signaling in tumor cells associates with primary resistance to anti-CTLA-4 therapy
show Abstracthide Abstract
"Antibody blockade of the inhibitory CTLA-4 pathway has led to clinical benefit in a subset of patients with metastatic melanoma. Anti-CTLA-4 enhances T cell responses, including production of IFN-?, which is a critical cytokine for host immune responses. However, the role of IFN-? signaling in tumor cells in the setting of anti-CTLA-4 therapy remains unknown. Here we demonstrate that, based upon exome sequencing data, patients identified as non-responders to anti-CTLA-4 (ipilimumab) harbor a much higher genomic defects in the IFN-? pathway genes than melanoma patients who had clinical response to ipilimumab therapy."
Sample: Non-tumor DNA sample from a human female participant in the dbGaP study "Genetic Analysis of Tumor Samples"
SAMN06631640 • SRS2087375 • All experiments • All runs
Organism: Homo sapiens
Library:
Name: N46
Instrument: Illumina HiSeq 2000
Strategy: WXS
Source: GENOMIC
Selection: Hybrid Selection
Layout: PAIRED
Spot descriptor:
forward77  reverse

Experiment attributes:
alignment_software: BWA
The SRA run(s) below contain human sequence (more...)(less...)

These data are available through the dbGaP authorized access system. Request access to:

  • Study:  phs001257
  • Consent Group: DS-M-PUB-NPU

Runs: 1 run, 60.2M spots, 9.2G bases, 4.3Gb
Run# of Spots# of BasesSizePublished
SRR539529560,238,1949.2G4.3Gb2017-04-12

ID:
3875219

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