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ERX2257907: Illumina HiSeq 2500 paired end sequencing
1 ILLUMINA (Illumina HiSeq 2500) run: 1.3M spots, 247M bases, 157.7Mb downloads

Design: ddRAD-seq using MseI and SbfI enzymes, AMPure1:1, no other size selection
Submitted by: Institute of Botany, Polish Academy of Sciences
Study: Phylogenomic study on the Chiastocheta genus (Diptera: Anthomyiidae) using RAD-sequencing
show Abstracthide Abstract
We examine the potential and limitations of RAD-sequencing in disentangling the phylogeny of the fly genus Chiastocheta (Diptera: Anthomyiidae). In Europe, this genus encompasses seven species of seed predators, which have been widely studied in the context of their ecological and evolutionary interactions with the plant Trollius europaeus (Ranunculaceae). So far, phylogenetic analyses using mitochondrial markers failed to resolve monophyly of most of the species from this genus. Contrasting with results based on mitochondrial markers, RAD-sequencing analyses retrieved the monophyly of all seven species, in agreement with the morphological species assignment.
Sample: I_RAD_31
SAMEA104399396 • ERS2017338 • All experiments • All runs
Library:
Name: I_RAD_31
Instrument: Illumina HiSeq 2500
Strategy: RAD-Seq
Source: GENOMIC
Selection: Restriction Digest
Layout: PAIRED
Construction protocol: Suchan et al. 2016
Runs: 1 run, 1.3M spots, 247M bases, 157.7Mb
Run# of Spots# of BasesSizePublished
ERR22035331,260,014247M157.7Mb2017-11-28

ID:
4772984

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