Microbe sample from Escherichia coli mutant Flu100ppm-30D chl-4 - SRA

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SRX3873985: Microbe sample from Escherichia coli mutant Flu100ppm-30D chl-4
1 OXFORD_NANOPORE (MinION) run: 31,245 spots, 251M bases, 212.1Mb downloads

Design: The sample was sequenced following the 1D genomic DNA selecting for long reads using SQK LSK108 protocol, which was similar to the 2D protocol, but with the use of Ampure XP beads instead of MyOne Streptavidin C1 beads after the ligation of the adaptors. The protocols were followed according to the manufacturer s instructions except for the following steps: incubation time of 20 minutes in each end repair and ligation steps, 0.7x AMPure XP beads for purification, and warm up elution buffer at 50 C.

Submitted by: University of Queensland-AWMC

Study: Escherichia coli str. K-12 substr. MG1655 Genome sequencing and assembly

PRJNA445757 • SRP136964 • All experiments • All runs

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We aim to investigate whether the exposure to chemical triclosan or fluoxetine has an impact on inducing antibiotic resistance on Escherichia coli. Treatment-induced antibiotic resistant mutants were selected and subjected to MinION nanopore sequencing to detect potential chromosome structure variations.

Sample: Escherichia coli K-12 MG1655 mutant Flu30-Chl4

SAMN08798398 • SRS3115134 • All experiments • All runs

Organism: Escherichia coli str. K-12 substr. MG1655

Library:

Name: S10

Instrument: MinION

Strategy: WGS

Source: GENOMIC

Selection: size fractionation

Layout: SINGLE

Runs: 1 run, 31,245 spots, 251M bases, 212.1Mb

Run	# of Spots	# of Bases	Size	Published
SRR6928157	31,245	251M	212.1Mb	2019-05-01

ID:: 5326461

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