GSM4631066: Zf_30h_trunk; Danio rerio; RNA-Seq - SRA

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SRX8595501: GSM4631066: Zf_30h_trunk; Danio rerio; RNA-Seq
2 ILLUMINA (Illumina HiSeq 2500) runs: 340.6M spots, 62G bases, 34.4Gb downloads

Submitted by: NCBI (GEO)

Study: Single-cell transcriptomic analysis 30 hpf Zebrafish trunks

PRJNA641114 • SRP268312 • All experiments • All runs

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Here we performed single-cell RNAseq analysis of whole wildtype zebrafish trunks at 30hpf using 10x Genomics' Chromium platform. 22 distinct cell populations were identified, spanning all three embryonic germ layers. Overall design: Whole trunks excised from 30 embryos at 30 hpf were dissociated using a cold protease dissociation protocol. Barcoding of cells and and cDNA library construction was carried out using 10X Genomics' Chromium platform.

Sample: Zf_30h_trunk

SAMN15340941 • SRS6886082 • All experiments • All runs

Organism: Danio rerio

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Whole wildtype zebrafish trunks at 30 hpf were dissociated into a single cell suspension using a cold protease treatment.Single-cell suspensions were processed through the Chromium platform (10X Genomics) to generate single-cell cDNA libraries cDNA was amplified by PCR, and the 3' end of the cDNA prepared for sequencing using a modified Nextera XT protocol

Experiment attributes:

GEO Accession: GSM4631066

Links:

NCBI link: NCBI Entrez (gds)

Runs: 2 runs, 340.6M spots, 62G bases, 34.4Gb

Run	# of Spots	# of Bases	Size	Published
SRR12067711	167,958,921	30.6G	17Gb	2020-06-23
SRR12067712	172,683,368	31.4G	17.5Gb	2020-06-23

ID:: 11173251

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