Instrument: PacBio RS
Strategy: OTHER
Source: GENOMIC
Selection: other
Layout: SINGLE
Construction protocol: EDTA (20 mM), proteinase K (50 μg mL-1), and sodium dodecyl sulfate (0.5%, w/v) were added to the purified phage stock solution (PaP1 or PaP3). The mixture was incubated at 56 °C for 1 h, then an equal volume of phenol–chloroform–isoamyl alcohol solution (25:24:1) was added, followed by centrifugation at 5,000 g for 10 min. The aqueous layer was collected and extracted with chloroform at 5,000 g for 10 min. Finally, the collected aqueous layer was mixed with 0.6 volumes of isopropanol, and stored overnight at –20 °C. The next day, the mixture was centrifuged for 10 min at 12,000 g and 4 °C, and the precipitated DNA was collected and washed with 70% and 100% ethanol, respectively. The PaP1 DNA was suspended in TE buffer (pH 8.0) and stored at –20 °C for subsequent use. Genomic DNA was randomly sheared to an avearge size of approximately 2,000 bp, end repaired, and ligated to blunt end hairpin adapters for form standard SMRTbell templates