All WGS libraries for E. macaco sample Harmonia (source for reference... - SRA

SRX1038508: All WGS libraries for E. macaco sample Harmonia (source for reference-based E. macaco assembly)
1 ILLUMINA (Illumina HiSeq 2000) run: 280M spots, 50.5G bases, 27.2Gb downloads

Design: PE libraries with mean target insert size of 500 bp were prepared with Illumina’s TruSeq DNaseq Sample prep kit at the Keck Sequencing Center of the University of Illinois (Champaign, IL); these libraries were also sequenced at the Keck Center. PE libraries were quantitated by qPCR and sequenced for 100 cycles from each end of the fragments on a HiSeq2000 machine using a TruSeq SBS sequencing kit version 3. Reads were analyzed with Casava 1.8 (pipeline 1.9) and subsequently stripped of adaptors. Duplicates were marked using Picard MarkDuplicates, and reads were mapped using used the recommended pipeline within BWA version 0.5.9 and locally around indels using GATK’s RealignerTargetCreator and IndelRealigner.

Submitted by: University of Chicago

Study: Whole genome sequence of Eulemur flavifrons and Eulemur macaco

PRJNA284191 • SRP058683 • All experiments • All runs

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Our lab has used next-generation sequencing technology to produce high coverage genome-wide sequence data for a single Eulemur flavifrons individual and a single Eulemur macaco individual, in order to address questions related to the demographic history of the two species, as well as to identify candidate regions that may influence their divergent pigmentation phenotypes.

Sample:

SAMN03699689 • SRS945363 • All experiments • All runs

Organism: Eulemur macaco

Library:

Name: EmHarmonia_alllibs

Instrument: Illumina HiSeq 2000

Strategy: WGS

Source: GENOMIC

Selection: unspecified

Layout: PAIRED

Spot descriptor:

1 forward101 reverse

Runs: 1 run, 280M spots, 50.5G bases, 27.2Gb

Run	# of Spots	# of Bases	Size	Published
SRR2040129	280,038,456	50.5G	27.2Gb	2015-06-02

ID:: 1505768

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Sequence Read Archive

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