Table 1Summary of Assays and AIDs

PubChemBioAssay NameAIDsProbe TypeAssay TypeAssay FormatAssay Detection & well format
Summary assay for the identification of chemical inhibitors of B-cell specific antigen receptor-induced NF-κB activation.[Summary]435025InhibitorSummaryN/AN/A
uHTS luminescence assay for the identification of chemical inhibitors of B-cell specific antigen receptor-induced NF-κB activation [Primary Screening]435022InhibitorPrimaryCell-basedLuminescence & 1536 well
Single concentration confirmation of chemical inhibitors of B-cell specific antigen receptor-induced NF-κB activation [Primary Screening]449746InhibitorConfirmatory (Cherry Pick)Cell-basedLuminescence & 1536 well
Dose response confirmation of uHTS of chemical inhibitors of B-cell specific antigen receptor-induced NF-κB activation in a 697B cell line using a luminescence assay [Confirmatory]489023InhibitorConfirmatory (DMSO Dose Response)Cell-basedLuminescence & 1536 well
SAR Analysis of small molecule inhibitors of B-cell specific antigen receptor-induced NF-κB activation in a 697B cell line using a luminescence assay [Confirmatory]504485; 504508InhibitorConfirmatory (Dry Powder)Cell-basedLuminescence & 1536 well
Dose response cytotoxicity of uHTS chemical inhibitors of B-cell specific antigen receptor-induced NF-κB activation in a HEK-293T cell line using a luminescence assay [Confirmatory]489021InhibitorSecondary (DMSO DR)Cell-basedFluorescence & 1536 well
SAR analysis of small molecule cytotoxicity in HEK293 cells for activators of NF-kappaB using a luminescence assay [Confirmatory]493251; 504528InhibitorSecondary (Dry Powder)Cell-basedFluorescence & 1536 well
Dose response counterscreen of uHTS chemical inhibitors of B-cell specific antigen receptor-induced NF-κB activation in a HEK-293T cell line using a luminescence assay [Confirmatory]489022InhibitorSecondary (DMSO DR)Cell-basedLuminescence & 1536 well
SAR Analysis of small molecule inhibitors of B-cell specific antigen receptor-induced NF-κB activation in a HEK-293T cell line using a luminescence assay [Confirmatory]504518InhibitorSecondary (Dry Powder)Cell-basedLuminescence & 1536 well
Dose Response selectivity of uHTS chemical inhibitors of B-cell specific antigen receptor-induced NF-κB activation in Jurkat cells using a luminescence assay [Confirmatory]489006InhibitorSecondary (DMSO DR)Cell-basedLuminescence & 1536 well
SAR Analysis of small molecule inhibitors of B-cell specific antigen receptor-induced NF-κB activation in a Jurkat cell line using a luminescence assay [Confirmatory]504516; 504515InhibitorSecondary (Dry Powder)Cell-basedLuminescence & 1536 well

From: B-cell Specific Inhibitors of NF-κB Activation

Cover of Probe Reports from the NIH Molecular Libraries Program
Probe Reports from the NIH Molecular Libraries Program [Internet].

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