Probe Reports from the NIH Molecular Libraries Program [Internet].
Bethesda (MD): National Center for Biotechnology Information (US); 2010-.
NCBI Bookshelf. A service of the National Library of Medicine, National Institutes of Health.
Table 3Tb PFK Kinase-Glo assay protocol
| Sequence | Parameter | Value | Description |
|---|---|---|---|
| 1 | Reagent | 3 μL | ATP/F6P substrate buffer (0.1 & 0.5mM final conc., resp.) |
| 2 | Compound | 23 nL | Compound Library |
| 3 | Reagent | 1 μL | Tb PFK (1.25nM final conc.) or buffer-only control |
| 4 | Time | 45 min | RT incubation |
| 5 | Reagent | 2 μL | Kinase-Glo Plus reagent |
| 6 | Time | 10 min | RT incubation |
| 7 | Detector | Luminescence | ViewLux in end-point mode: 1 second exp. |