GEO DataSets

(Submitter supplied) The main objective of this study was to analyze the general midgut transcript profile of Schiustocerca gregaria as well as changes in midgut gene expression during different stages of feeding. We compared three different time points of the digestive process: no active digestion (24 h after feeding), the initial stages of feeding (10 min after feeding) and active digestion in the midgut (2 h after feeding).

Organism:

Schistocerca gregaria

Type:

Expression profiling by high throughput sequencing

Platform:

GPL33225

18 Samples

Download data: FASTA, RESULTS

(Submitter supplied) a high-throughput Illumina/Solexa sequencing was conducted, 478262, 702593, 835394 and 894277 unique sRNAs was discovered from four periods of M. robertsii infection, uninfected (0h), infected for 12h, 24h and 36h. Then, 7, 7, 6 and 7 known pre-miRNAs were obtained, and 33, 58, 54 and 52 candidate novel miRNAs was detected in four periods. Further analysis showed that 24 of those candidate novel miRNAs were matched to other known insect miRNAs, while 36 of those miRNAs lacked sequence homologues of insect organisms.

Organism:

Locusta migratoria

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL23516

4 Samples

Download data: TXT

(Submitter supplied) Using hight throughput sequencing and data processing, we identified miRNA and quantified their expression in the fat body of adult females of Locusta migratoria.

Organism:

Locusta migratoria

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL25868

12 Samples

Download data: XLSX

(Submitter supplied) The transcriptome and DGE analysis of the fat body and ovary of L. migratoria based on the Illumina short-read sequencing technology and De novo assembly. Research on the trade-offs between immunity and reproduction is contributing significantly to the understanding of the fitness of organisms in nature.

Organism:

Locusta migratoria

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18662

12 Samples

Download data: FASTA, TXT

(Submitter supplied) DNA methylation is a widely conserved epigenetic modification that is established and maintained by the cooperative activity of DNA methyltransferases. While the complement of DNA methyltransferase genes can vary substantially between animal species, whole-genome methylation analyses have suggested that major features of animal methylomes are widely conserved. We have now used genome-scale bisulfite sequencing to analyze the methylome of the desert locust, Schistocerca gregaria, which represents an economically important pest with a high degree of phenotypic plasticity. more...

Organism:

Schistocerca gregaria

Type:

Methylation profiling by high throughput sequencing

Platform:

GPL16114

2 Samples

Download data: BEDGRAPH

(Submitter supplied) We report the application of single-molecule-based sequencing technology for high-throughput profiling of nervous system in locust Locusta migratoria manilensis. By obtaining over 57,000,000 bases of sequence from central nervous system, we generated 101836 contigs and 69440 scaffolds. We finally get 41179 unigene with an average length of 570bp. There are 5519 unigenes beyond the length of 1000bp. Using BLAST searches of the NR, NT, Swiss-Prot, KEGG and COG databases we are able to identify 13552 unigene (E<0.0001). Comprehensive assessment of all the unigenes by comparing with the studied genes of other insects nervous system reveals that our unigene are broadly representative of the transcriptome of insect nervous system. Our data provides the most large-scale EST-project for locust nervous system, which greatly benefits the exploring of this insect. In addition, we identify a large number of novel nervous genes which can be used in systematic studies of locust and other insects.

Organism:

Locusta migratoria manilensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11245

1 Sample

Download data: FA

(Submitter supplied) All the reports on insect small RNAs come from holometabolous insects. However, small RNAs of hemimetabolous insects have not yet been investigated.Study of hemimetabolous insect small RNAs could provide more insights into evolution and function of small RNAs in hemi- and holometabolous insects. The locust is an important, economically harmful hemimetabolous insect and its phase changes is an interesting phenomenon.Here, we used high-throughput sequencing to characterize and compare the small RNA transcriptomes of gregarious and solitary phases in locusts. more...

Organism:

Locusta migratoria

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL9308

2 Samples

Download data