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| Status |
Public on Sep 13, 2023 |
| Title |
Differential midgut transcriptomics comparing nutritional status reveals a crucial role for V-ATPase subunit a and Niemann-pick 1b protein in the digestive physiology of the desert locust. |
| Organism |
Schistocerca gregaria |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The main objective of this study was to analyze the general midgut transcript profile of Schiustocerca gregaria as well as changes in midgut gene expression during different stages of feeding. We compared three different time points of the digestive process: no active digestion (24 h after feeding), the initial stages of feeding (10 min after feeding) and active digestion in the midgut (2 h after feeding).
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| Overall design |
Samples are RNA extracts of the desert locust midgut. We compared 3 timepoints. Each timepoint consists of 6 biological replicates.
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| Contributor(s) |
Van Lommel J, Holtof M, Cools D, Vansteenkiste S, Polgun D, Tilleman L, Van Nieuwerburgh F, Vanden Broeck J |
| Citation(s) |
37692997 |
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| Submission date |
Mar 07, 2023 |
| Last update date |
Sep 14, 2023 |
| Contact name |
Laurentijn Tilleman |
| Organization name |
Ghent University
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| Street address |
Ottergemsesteenweg 460
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| City |
Gent |
| ZIP/Postal code |
9000 |
| Country |
Belgium |
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| Platforms (1) |
| GPL33225 |
Illumina NextSeq 500 (Schistocerca gregaria) |
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| Samples (18)
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| GSM7086179 |
Midgut, 10 minutes after feeding, replicate 1 |
| GSM7086180 |
Midgut, 10 minutes after feeding, replicate 2 |
| GSM7086181 |
Midgut, 10 minutes after feeding, replicate 3 |
| GSM7086182 |
Midgut, 10 minutes after feeding, replicate 4 |
| GSM7086183 |
Midgut, 10 minutes after feeding, replicate 5 |
| GSM7086184 |
Midgut, 10 minutes after feeding, replicate 6 |
| GSM7086185 |
Midgut, 2 hours after feeding, replicate 1 |
| GSM7086186 |
Midgut, 2 hours after feeding, replicate 2 |
| GSM7086187 |
Midgut, 2 hours after feeding, replicate 3 |
| GSM7086188 |
Midgut, 2 hours after feeding, replicate 4 |
| GSM7086189 |
Midgut, 2 hours after feeding, replicate 5 |
| GSM7086190 |
Midgut, 2 hours after feeding, replicate 6 |
| GSM7086191 |
Midgut, 24 hours after feeding, replicate 1 |
| GSM7086192 |
Midgut, 24 hours after feeding, replicate 2 |
| GSM7086193 |
Midgut, 24 hours after feeding, replicate 3 |
| GSM7086194 |
Midgut, 24 hours after feeding, replicate 4 |
| GSM7086195 |
Midgut, 24 hours after feeding, replicate 5 |
| GSM7086196 |
Midgut, 24 hours after feeding, replicate 6 |
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| Relations |
| BioProject |
PRJNA941911 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE226871_RAW.tar |
25.4 Mb |
(http)(custom) |
TAR (of RESULTS) |
| GSE226871_reference_transcriptome.fasta.gz |
23.9 Mb |
(ftp)(http) |
FASTA |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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