GEO DataSets

(Submitter supplied) Isolated islets used for transplantation are known to be stressed, which can result from the circumstances of death, in particular brain dead, the preservation of the pancreas with its warm and cold ischemia, from the trauma of the isolation process, and the complex events that occur during culture tissue. Isolated islets used for transplantation are known to be stressed, which can result from the circumstances of death, in particular brain death, the preservation of the pancreas with its warm and cold ischemia, from the trauma of the isolation process, and the complex events that occur during tissue culture. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1352

14 Samples

Download data: CEL

(Submitter supplied) The heterotransplantation of human tumors to immune-deprived rodents has become an important preclinical tool for evaluating human tumor biology and responsiveness to therapeutic agents, based on the supposition that they reflect their original human origin. However, we demonstrate that such tumors can in fact change genetically, either by becoming tumors of the animal recipient or by becoming a hybrid of the human tumor and the animal host’s cells. more...

Organism:

Mesocricetus auratus; Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1352

5 Samples

Download data: CEL, CHP

(Submitter supplied) The use of nucleic acids from formalin-fixed paraffin-embedded (FFPE) tissues for high-throughput molecular techniques, such as microarray gene expression profiling has become widespread in molecular research area. However, working with FFPE tissues is challenging because of degradation, cross-linking with proteins, and RNA chemical modifications. Also, there is no generally accepted procedure for RNA extraction to microarray analysis. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL570 GPL1352

19 Samples

Download data: CEL, CHP

(Submitter supplied) Purpose: There is an unmet clinical need for biomarkers to identify breast cancer patients who are at increased risk of developing brain metastases. The objective is to identify gene signatures and biological pathways associated with HER2+ brain metastasis. Experimental Design: Gene expression of 19 HER2+ breast cancer brain metastases was compared with HER2+ nonmetastatic primary tumors. Gene Set Enrichment Analysis was used to identify a signature, which was evaluated for correlation with BRCA1 mutation status and clinical outcome using published microarray datasets and for correlation with pharmacological inhibition by a PARP inhibitor and temozolomide using published microarray datasets of breast cancer cell lines. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5306

Platform:

GPL1352

38 Samples

Download data: CEL

(Submitter supplied) Dysregulation of pyramidal cell network function by the soma- and axon-targeting inhibitory neurons that contain the calcium-binding protein parvalbumin (PV) represents a core pathophysiological feature of schizophrenia. In order to gain insight into the molecular basis of their functional impairment, we used laser capture microdissection (LCM) to isolate PV-immunolabeled neurons from layer 3 of Brodmann’s area 42 of the superior temporal gyrus (STG) from postmortem schizophrenia and normal control brains. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1352

16 Samples

Download data: CEL, CHP

(Submitter supplied) The objective of this study was to determine how TNF-a, an important proinflammatory cytokine, affects gene expression in the human annulus. Cells were grown in a 3D collagen construct for 14 days with TNF-a. mRNA was isolated and subjected to microarray. Fold changes in gene expression were determined via GeneSifer software.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1352

8 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL8300 GPL1352

126 Samples

Download data: CEL

(Submitter supplied) We used gene expression profiling of human DCIS and IBC to discover uniquely expressed genes that may also regulate progression.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1352

22 Samples

Download data: CEL, TXT

(Submitter supplied) We used gene expression profiling of human DCIS and IBC to discover uniquely expressed genes that may also regulate progression.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1352

22 Samples

Download data: CEL, TXT

(Submitter supplied) Although the degenerating disc is considered to be the key source of pain in patients with low back pain, the relationship between disc cells, nerves, and pain production is poorly understood. Neurotrophins are signaling molecules involved in the survival, differentiation, migration, and neurite outgrowth of central and peripheral neurons. Neurotrophins are now known to be expressed in non-neuronal tissues, including the intervertebral disc. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1352

8 Samples

Download data: CEL

(Submitter supplied) Gray matter volume in the cerebral cortex has been consistently found to be decreased in patients with schizophrenia. The superior temporal gyrus (STG) is one of the cortical regions that exhibit the most pronounced volumetric reduction. This reduction is generally thought to reflect, at least in part, decreased number of synapses; the majority of these synapses are believed to be furnished by glutamatergic axon terminals onto the dendritic spines on pyramidal neurons. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1352

18 Samples

Download data: CEL, CHP

(Submitter supplied) Although it is well-recognized that apoptosis, senescence, and increased production of inflammatory cytokines and catabolic products are important factors in the degeneration of the human intervertebral disc, there is poor understanding of the underlying cause. The objective of the present study was to analyze gene expression patterns in the human annulus disc tissue.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1352

23 Samples

Download data: CEL

(Submitter supplied) Analysis of human dopamine (DA) from postmortem brains of 8 patients with Parkinson’s disease (PD). Results provide insight into the molecular processes perturbed in the PD substantia nigra.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1352

17 Samples

Download data: CEL

(Submitter supplied) Islet transplantation exposes beta cells to mild hyperglycemia and to the abnormal environment of the transplant site. These conditions may affect beta cells and induce the expression of genes involved in beta cell damage. Gene expression profile of human beta cells exposed to mild hyperglycemia by transplantation into ICR-SCID mice was evaluated and compared with the gene profile of beta cells obtained from non-diabetic subjects. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1352

13 Samples

Download data: CEL

(Submitter supplied) Most individuals infected with Mycobacterium tuberculosis can control the infection by forming and maintaining TB granulomas at the local infection foci. However, when the chronic infection (also known as latency) becomes active, the caseous center of TB granuloma enlarges, and it liquefies and cavitates, ultimately releasing bacilli into airway. Deciphering how genes are regulated within TB granulomas will help to understand the granuloma biology. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4256

Platform:

GPL1352

7 Samples

Download data: CEL, CHP, TXT

(Submitter supplied) Changes in gene expression in pancreatic beta-cells from type 2 diabetes could provide insights into their abnormal insulin secretion and beta-cell turnover. The laser capture microdissection technique was used to acquire beta-cells from pancreatic tissue sections obtained from type 2 diabetic (T2D) and non-diabetic controls. We found that 4% of analyzed transcripts were differentially expressed between the two groups at the lower confidence bound cutoff of 1.2, and, among the differentially expressed transcripts, 62% were up-regulated and 38% down-regulated in samples of T2D subjects compared to non-diabetic controls. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3782

Platform:

GPL1352

20 Samples

Download data: CEL

(Submitter supplied) Autologous stem cell therapy has potential for biologic treatment of disc degeneration. Due to ease of harvest and abundance, adipose-derived mesenchymal stem cells (AD-MSC) are readily available. Our objectives were: 1) To develop/validate methods to harvest AD-MSC and direct them to a disc-like phenotype by three-dimensional (3D) culture and TGF-ß3 exposure; 2) To perform gene expression profiling for human AD-MSC and annulus cells in 3D culture; 3) To test whether disc cell-AD-MSC co-culture could augment proteoglycan production. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1352

13 Samples

Download data: CEL, CHP

(Submitter supplied) The presence of senescent cells in the aging/degenerating human disc is now well-recognized. Senescent cells are viable, cannot divide, remain metabolically active and accumulate within the disc over time. Molecular analysis of senescent cells in tissue, however, offers a special challenge since there are no cell surface markers for senescence which would let one use fluorescence-activated cell sorting as a method for separating out senescent cells. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1352

19 Samples

Download data: CEL, CHP

(Submitter supplied) Asporin, also known as periodontal ligament-associated protein 1 (PLAP1), is a member of the family of small leucine-rich proteoglycan (SLRP) family. It is present within the cartilage extracellular matrix (ECM), and is reported have a genetic association with osteoarthritis. Its D14 allele has recently been found to be associated with lumbar disc degeneration in Asian subjects. There have been no studies, however, of this gene’s normal immunohistochemical localization within the human intervertebral disc, nor of expression levels in Caucasian individuals with disc degeneration. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1352

15 Samples

Download data: CEL, CHP

(Submitter supplied) K-Ras mutations occur frequently in epithelial cancers. Using shRNAs to deplete K-Ras in lung and pancreatic cancer cell lines harboring K-Ras mutations, two classes were identified—lines that do or do not require K-Ras to maintain viability. Comparing these two classes of cancer cells revealed a gene expression signature in K-Ras-dependent cells, associated with a well-differentiated epithelial phenotype, which was also seen in primary tumors. more...

Organism:

Homo sapiens

Type:

Expression profiling by array; Genome binding/occupancy profiling by SNP array

Platforms:

GPL1352 GPL3720 GPL3718

60 Samples

Download data: CEL, TXT