GEO DataSets

(Submitter supplied) Please refer to the summary of the Superseries.

Organism:

Human adenovirus 2

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30034

5 Samples

Download data: SHAPE

(Submitter supplied) Please refer to the summary of the Superseries.

Organism:

Human adenovirus 2

Type:

Other

Platform:

GPL30034

6 Samples

Download data: TXT

(Submitter supplied) Please refer to the summary of the Superseries.

Organism:

Human adenovirus 2

Type:

Other

Platform:

GPL30034

11 Samples

Download data: SHAPE

(Submitter supplied) We recently reported that serine-arginine-rich (SR) protein-mediated pre-mRNA structural remodeling generates a pre-mRNA 3D structural scaffold that is stably recognized by the early spliceosomal components. However, the intermediate steps between the free pre-mRNA and the assembled early spliceosome are not yet characterized. By probing the early spliceosomal complexes in vitro and RNA-protein interactions in vivo, we show that the SR proteins bind the pre-mRNAs cooperatively generating a substrate that recruits U1 snRNP and U2AF65 in a splice signal-independent manner. more...

Organism:

Human adenovirus 2

Type:

Other

Platform:

GPL30034

22 Samples

Download data: SHAPE, TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Human adenovirus 2

Type:

Other

Platform:

GPL30034

59 Samples

Download data

(Submitter supplied) The specific recognition of splice signals at or near the exon-intron junctions is not explained by their weak conservation across the mammalian transcriptome and postulated to require a multitude of features embedded in the pre-mRNA strand. We explored the possibility of three-dimensional structural scaffold of a pre-mRNA guiding early spliceosomal components to the splice signal sequences. We find that mutation in non-cognate splice signal sequences of a model pre-mRNA substrate could impede recruitment of early spliceosomal components due to disruption of global structure of the pre-mRNA. more...

Organism:

Human adenovirus 2

Type:

Other

Platform:

GPL30034

9 Samples

Download data: SHAPE

(Submitter supplied) The specific recognition of splice signals at or near exon-intron junctions is not explained by their weak conservation and instead is postulated to require a multitude of features embedded in the pre-mRNA strand. We explored the possibility of three-dimensional structural scaffold of AdML – a model pre-mRNA substrate – guiding early spliceosomal components to the splice signal sequences. We find that mutations in the non-cognate splice signal sequences impede recruitment of early spliceosomal components due to disruption of the global structure of the pre-mRNA. more...

Organism:

Human adenovirus 2

Type:

Other

Platform:

GPL30034

50 Samples

Download data: SHAPE