GEO DataSets

(Submitter supplied) Blastocladiomycota, early diverging zoosporic (flagellated) lineages of fungi, are vastly understudied. This phylum includes the genus Coelomomyces which consists of more than eighty fungal species that are obligate parasites of arthropods. Coelomomyces lack a complete asexual life cycle, instead surviving through an obligate heteroecious alternation of generations life cycle. Despite their global distribution and interesting life cycle, little is known about Coelomomyces species. more...

Organism:

Coelomomyces lativittatus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL33004

14 Samples

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(Submitter supplied) Blastocladiella emersonii life cycle presents a number of drastic biochemical and morphological changes, mainly during two cell differentiation stages: the germination and the sporulation. To investigate the transcriptional changes taking place during the sporulation phase, which culminates with the production of the zoospores, motile cells responsible for the dispersal of the fungus, microarray experiments were performed. more...

Organism:

Blastocladiella emersonii

Type:

Expression profiling by array

Platform:

GPL4516

57 Samples

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(Submitter supplied) Due to its aquatic saprophytic lifestyle, the fungus Blastocladiella emersonii seems to be adapted to low aerated environments and thus can be an interesting model of study of the hypoxic stress response. Iron deprivation, a hypoxia-mimicking stimulus due to HIF-1 stabilization, and geldanamycin, that causes HIF-1 depletion by HSP90 inhibition, are helpful parameters to find an evidence of an oxygen-dependent regulatory mechanism in B. more...

Organism:

Blastocladiella emersonii

Type:

Expression profiling by array

Platform:

GPL4516

80 Samples

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(Submitter supplied) To investigate global changes in gene expression during B. emersonii germination process, we constructed cDNA microarrays containing 3,563 putative unique genes. Analyses were carried out during germination induced under various environmental conditions. Microarray data revealed that 26% of them are differentially expressed during germination in nutrient medium, in at least one of the time points investigated. more...

Organism:

Blastocladiella emersonii

Type:

Expression profiling by array

Platform:

GPL7334

42 Samples

Download data: TXT

(Submitter supplied) We explored transcriptional responses of the chytridiomycete Blastocladiella emersonii to some stress environmental conditions. Three distinct cDNA libraries constructed from mRNAs extracted from cells submitted to heat shock and different cadmium concentrations (50 and 100 mM) were sequenced and generated 6,350 high-quality EST sequences. These ESTs were assembled into 2,326 unigenes, being 51% of them not previously described in B. more...

Organism:

Blastocladiella emersonii

Type:

Expression profiling by array

Platform:

GPL4516

42 Samples

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(Submitter supplied) The array consists of cDNA inserts from 3,563 distinct B. emersonii expressed genes obtained through Georg and Gomes (2007) and Ribichich et al. (2005) works. These inserts were PCR amplified with the T7 forward and SP6 reverse primers. The amplified products were purified in Millipore Multiscreen Filtration Plates (MAFB NOB), eluted with Tris-HCl 10 mM, pH 8.0 to a final concentration of 100 to 200 ng/μl and spotted onto Star 7 slides (Amersham Biosciences) using a Generation III Microarray SpotterTM (Amersham Biosciences).

Organism:

Blastocladiella emersonii

1 Series

42 Samples

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(Submitter supplied) The array consists of cDNA inserts from representatives ESTs from 3,773 expressed genes that were obtained through Georg and Gomes, 2007 and Ribichich et al (2005) work. These inserts were PCR amplified with the T7 forward and SP6 reverse primers, and the amplified products were purified with a Millipore Multiscreen Filtration Plate (MAFB NOB), eluted with Tris-HCl 10 mM, pH 8.0 to a final concentration of 100 to 200 ng/μl and spotted onto Star 7 slides (Amersham Biosciences) using a Generation III Microarray SpotterTM (Amersham Biosciences).

Organism:

Blastocladiella emersonii

3 Series

179 Samples

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