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| Status |
Public on Jan 21, 2023 |
| Title |
Genomic resources enable study of the blastoclad fungus, Coelomomyces lativittatus, an obligate parasite of mosquitoes and microcrustaceans, during infection and sporulation |
| Organism |
Coelomomyces lativittatus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Blastocladiomycota, early diverging zoosporic (flagellated) lineages of fungi, are vastly understudied. This phylum includes the genus Coelomomyces which consists of more than eighty fungal species that are obligate parasites of arthropods. Coelomomyces lack a complete asexual life cycle, instead surviving through an obligate heteroecious alternation of generations life cycle. Despite their global distribution and interesting life cycle, little is known about Coelomomyces species. To begin to address this, we analyzed transcriptomes from across host-associated life stages including infection of larva and excised mature sporangia from the mosquito, Anopheles quadrimaculatus. We identified differentially expressed genes and over-enriched GO terms both across and within life stages and used these to make hypotheses about C. lativittatus biology. Generally, we found the C. lativittatus transcriptome to be a complex and dynamic expression landscape; GO terms related to metabolism and transport processes were over-enriched during infection and terms related to dispersal were over-enriched during sporulation. The C. lativittatus transcriptomes reported here are a valuable resource and may be leveraged toward furthering understanding of the biology of these and other early diverging fungal lineages.
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| Overall design |
Comparative gene expression profiling analysis of RNA-seq data for Coelomomyces lativittatus developmental stages
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| Contributor(s) |
Ettinger C, Ostovar T, Yacoub M, Ahrendt S, Hice R, Federici B, Stajich JE |
| Citation missing |
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| |
| Submission date |
Jan 05, 2023 |
| Last update date |
Jan 21, 2023 |
| Contact name |
Jason E Stajich |
| E-mail(s) |
jason.stajich@ucr.edu
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| Organization name |
University of California, Riverside
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| Department |
Microbiology & Plant Pathology
|
| Street address |
900 University Ave
|
| City |
Riverside |
| State/province |
CA - California |
| ZIP/Postal code |
92521 |
| Country |
USA |
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| Platforms (1) |
| GPL33004 |
Illumina HiSeq 4000 (Coelomomyces lativittatus) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA918605 |
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