GEO DataSets

Analysis of embryonic stem cells differentiated in the presence of PD169316, an inhibitor of p38 that blocks apoptosis without altering differentiation. Results identify gene families whose transcriptional regulation correlates with cell fate: differentiation with or without apoptosis.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent, 2 cell line sets

Platform:

GPL32

Series:

GSE2042

6 Samples

Download data: CEL, EXP

(Submitter supplied) Mouse embryonic stem (ES) cells remain pluripotent in vitro when grown in presence of Leukaemia Inhibitory Factor (LIF). LIF starvation leads to apoptosis of some of the ES-derived differentiated cells, together with p38a MAP kinase activation. Apoptosis, but not morphological cell differentiation, is blocked by a p38 inhibitor, PD 169316. To further understand the mechanism of action of this compound, we have identified its specific targets by microarray studies. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS1593 GDS1596

Platforms:

GPL32 GPL81

13 Samples

Download data: CEL, EXP

Analysis of embryonic stem cells differentiated in the presence of PD169316, an inhibitor of p38 that blocks apoptosis without altering differentiation. Results identify gene families whose transcriptional regulation correlates with cell fate: differentiation with or without apoptosis.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent sets

Platform:

GPL81

Series:

GSE2042

4 Samples

Download data: CEL, EXP

(Submitter supplied) time-course experiment with embryoid bodies of CGR8 mouse embryonic stem cells ; in the whole time-series RNA from 0 days old embryoid bodies were hybridized against RNA from 3 days and 10 days old embryoid bodies Keywords = embryoid bodies Keywords = mouse Keywords = time-course Keywords = oligonucleotide array Keywords: time-course

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1882

Platform:

GPL1942

4 Samples

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Analysis of CGR8 embryoid bodies differentiating for 3 or 10 days. Culturing of most embryonic stem (ES) cells require embryonic fibroblast (MEF) feeder cells which may affect their gene expression profiles. CGR8 cells are MEF-independent and represent a powerful model of ES cell differentiation.

Organism:

Mus musculus

Type:

Expression profiling by array, log2 ratio, 2 time sets

Platform:

GPL1942

Series:

GSE2469

4 Samples

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(Submitter supplied) The goal of this study was to identify the target genes of Stat3 involved in murine ES cell self-renewal. To achieve this goal, we used the Gs2 ES cell line expressing an inducible dominant negative mutant of Stat3 (Stat3F) in wich Y705 is mutated to phenilalanine. The Gs2 ES cells are routinely maintained in the presence of LIF 1000 U/ml and tetracycline 1µg/ml (LIF/ON). In this condition, Stat3F is not expressed and the cells are maintained in an undifferentiated phenotype. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platforms:

GPL1063 GPL1091

23 Samples

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(Submitter supplied) In order to identify the genes involved in LIF/gp130 self-renewal response, we analyzed the transcriptome of Gs2 ES cells induced to differentiate upon LIF withdrawal for 16h, 24h and 48h (LIF16, LIF24 and LIF48 respectively)and compared them to that of undifferentiated ES Gs2 cells continuously maintained in the presence of LIF. Since this cell line also carry a tetracycline regulatable dominant negative form of Stat3 (Stat3F, all the experiment was done in the presence of Tetracycline (tet ON) to avoid Stat3F expression. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1091

18 Samples

Download data

(Submitter supplied) Chip contains 9216 features (probes and controls): 2014 PCR products from cDNA matrix using primers specific of genes involved in important biological process (apoptosis, cell cycle, stress response). 1684 PCR products from cDNA clones from the I.M.A.G.E consortium (Research Genetics collection). 2014 PCR products from mouse cDNA clones of a substractive bank (myodistrophic versus normal muscle). 1800 PCR products from rat cDNA clones of a substractive bank (atrophied versus normal muscle). more...

Organism:

Mus musculus

5 Series

107 Samples

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(Submitter supplied) SAGE libraries from mouse

Organism:

Mus musculus

Type:

Expression profiling by SAGE

Platform:

GPL11

5 Samples

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(Submitter supplied) During development of the mammalian central nervous system (CNS), neurons and glial cells (astrocytes and oligodendrocytes) are generated from common neural precursor cells (NPCs). However, neurogenesis precedes gliogenesis, which normally commences at later stages of fetal telencephalic development. Astrocyte differentiation of mouse NPCs at embryonic day (E) 14.5 (relatively late gestation) is induced by activation of the transcription factor STAT3, whereas at E11.5 (mid-gestation) NPCs do not differentiate into astrocytes even when stimulated by STAT3-activating cytokines such as leukemia inhibitory factor (LIF). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

4 Samples

Download data: CEL

(Submitter supplied) DNA microarray analysis was performed with mouse multipotent adult germline stem cells (maGSCs) and embryonic stem cells (ESCs) from different genetic backgrounds cultured under standard ESC culture conditions and under differentiation-promoting conditions by the withdrawal of Leukemia Inhibitory Factor (LIF) and treatment with Retinoic Acid (RA). The analyzed undifferentiated cell lines are very similar based on their global gene expression pattern and show 97-99% identity dependent on the analyzed background. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4170

Platform:

GPL6246

18 Samples

Download data: CEL, CHP

Analysis of multipotent adult germline stem cells (maGSCs) and embryonic stem cells (ESCs) from different genetic backgrounds and under differentiation-promoting conditions. Results enable expression pattern comparisons between maGSCs and ESCs.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 cell type, 2 growth protocol, 2 strain sets

Platform:

GPL6246

Series:

GSE22605

18 Samples

Download data: CEL, CHP

(Submitter supplied) Embryonic stem (ES) cells can self-renew indefinitely without losing their differentiation ability to any cell types. Phosphoinositide-3 kinase (PI3K)/Akt signaling plays a pivotal role in various stem cell systems, including the formation of embryonic germ (EG) cells from primordial germ cells and self-renewal of neural stem cells. Here, we show that myristoylated, active form of Akt (myr-Akt) maintained the undifferentiated phenotypes in mouse ES cells without the addition of leukemia inhibitory factor (LIF). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1616

Platform:

GPL81

4 Samples

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Analysis of embryonic stem (ES) cells expressing a myristoylated, active form of Akt (myr-Akt) kinase. Myr-Akt expression maintains pluripotency of ES cells even in the absence of leukemia inhibitory factor (LIF). Results demonstrate Akt signaling is sufficient to maintain ES cell pluripotency.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent, 2 cell line sets

Platform:

GPL81

Series:

GSE2516

4 Samples

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(Submitter supplied) Pluripotent stem cells have been shown to have unique nuclear properties, e.g., hyperdynamic chromatin and large, condensed nucleoli. However, the contribution of the latter unique nucleolar character to pluripotency has not been well understood. Here, we show fibrillarin (FBL), a critical methyltransferase for ribosomal RNA (rRNA) processing in nucleoli, as one of the proteins highly expressed in pluripotent embryonic stem (ES) cells. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL7202

4 Samples

Download data: TXT

(Submitter supplied) Ablation of expression of the Max gene encoding a Myc protein partner in ES cells provoked two major phenomena, i.e. loss of pluripotency and apoptotic cell death. We found that nicotinamide (Nam) significantly alleviates these Max expression ablation-coupled phenotypes in ES cells. To see the alleviation effect of Nam on the overall expression profile of Max-null ES cells whose Max expression is controlled by the tet-off system, we eliminated Max expression by adding doxycycline (Dox) in the presence of Nam.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

4 Samples

Download data: CEL

(Submitter supplied) The goal of this study was to identify genes that are differentially expressed after genetic deletion of both alleles of the Cyp26a1 gene in murine embryonic stem cells. Cyp26a1 codes for the CYP26A1 enzyme which metabolizes RA to polar RA metabolites, such as 4-oxo-RA and 4-OH-RA. CYP26A1-/- ES cells do not metabolize RA within 48 hours of RA treatment while in Wt ES cells, polar RA metabolites are already detectable by 8 hr. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

18 Samples

Download data: CEL, CHP

(Submitter supplied) Transcriptional profiling of mouse embryonic stem cells comparing control untreated ES cells with ES cells transfected with a pUb-fluc-mrfp-ttk triple fusion reporter gene. The latter makes ES visualization possible by FACS and single cell microscopy, high throughput bioluminescence imaging, and PET. Goal was to determine the effects of triple-fusion (TF) gene transfection on global ES gene expression. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2187

Platform:

GPL1307

7 Samples

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Analysis of embryonic stem (ES) cells stably-transduced with a triple-fusion (TF) construct consisting of fluorescence, bioluminescence, and positron emission tomography reporter genes. Results provide insight into the feasibility of using the TF construct to track cells in vivo.

Organism:

Mus musculus

Type:

Expression profiling by array, log ratio, 2 protocol sets

Platform:

GPL1307

Series:

GSE3432

7 Samples

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(Submitter supplied) In order to identify the effects of the induction of the gene of interest on the mouse ES transcriptome, we performed Affymetrix Gene-Chip hybridization experiments for the different inducible cell lines Transcriptome analysis of the inducible transgenic mouse ES cell lines

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

51 Samples

Download data: CEL