GEO DataSets

(Submitter supplied) Microarray analysis of PBMC from cynomolgus macaques collected longitudinally over the course of infection with Lassa-Josiah, Lassa-Z132, Lassa-SorombaR, or Lujo viruses (n=3 animals/infection condition).

Organism:

Macaca mulatta; Macaca fascicularis

Type:

Expression profiling by array

Platform:

GPL14569

61 Samples

Download data: TXT

(Submitter supplied) Lassa fever (LF) is a rodent-borne viral disease that can be fatal for human beings. In this study, an attenuated Lassa vaccine candidate, ML29, was tested in SIV-infected rhesus macaques for its ability to elicit immune responses without instigating signs of virulent disease. ML29 is a reassortant between Lassa and Mopeia viruses that causes a transient infection in non-human primates and confers sterilizing protection from lethal Lassa viral challenge. more...

Organism:

Macaca mulatta; Homo sapiens

Type:

Expression profiling by array; Third-party reanalysis

Platform:

GPL570

30 Samples

Download data: CEL

(Submitter supplied) Lassa fever virus is a zoonotic pathogen that plagues the endemic areas of West Africa. Rhesus macaques infected with a related arenavirus, LCMV-WE, serve as a model for Lassa-infection of human beings. Using a dose similar to that expected from a needle-stick, monkeys experience an early pre-viremic phase (day 1-3), a viremic phase with febrile onset (day 4-7), and, like human beings who succumb, they die within two weeks. more...

Organism:

Macaca mulatta; Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

24 Samples

Download data: CEL

(Submitter supplied) Lassa fever virus (LASV) is a significant human pathogen that is endemic to several countries in West Africa. Infection with Lassa leads to the development of hemorrhagic fever in a significant number of public health cases and it is considered a potential bioweapon. Little is known about the complex immune mechanisms governing response to LASV infection, or the genetic determinants of susceptibility and resistance to infection. more...

Organism:

Homo sapiens; Macaca fascicularis

Type:

Expression profiling by array

Platform:

GPL4133

30 Samples

Download data: TXT

(Submitter supplied) The virulent Lassa fever virus (LASV) and the non-pathogenic Mopeia virus (MOPV) infect rodents and incidentally people in West Africa. The mechanism of LASV damage in human beings is unclear. A live-attenuated reassortant of MOPV and LASV protects rodents and primates from Lassa fever disease. Peripheral blood mononuclear cells from healthy human subjects were expose to either LASV or ML29 in order to identify early cellular responses that could be attributed to the difference in virulence between both viruses. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5058

Platform:

GPL570

27 Samples

Download data: CEL

Analysis of PBMCs exposed in vitro to LASV or ML29 viruses and collected up to 24 hrs post-exposure. The ML29 vaccine candidate is derived from Lassa and Mopeia viruses and protects against Lassa fever disease. Results provide insight into LASV-induced pathology and mechanisms of attenuation.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 3 dose, 3 protocol sets

Platform:

GPL570

Series:

GSE41300

27 Samples

Download data: CEL

(Submitter supplied) Marburg virus is a genetically simple RNA virus that causes a severe hemorrhagic fever upon infection in humans and non-human primates. The mechanism of how this pathogenesis comes about is not well understood, but it is well accepted that pathogenesis is significantly driven by a hyperactive immune response. To better understand the overall response to Marburg virus challenge, we undertook a transcriptomic analysis of immune cells circulating in the blood following aerosol exposure of cynomolgus macaques to a lethal dose of Marburg virus. more...

Organism:

Homo sapiens; Macaca fascicularis

Type:

Expression profiling by array

Platform:

GPL10332

30 Samples

Download data: TXT

(Submitter supplied) Lassa fever is a major threat in Western Africa. The large number of people living at risk for this disease calls for the development of a vaccine against Lassa virus (LASV). We compared the efficacy of measles-based and Mopeia-based vaccine platforms against LASV in cynomolgus monkeys. The vaccines were well tolerated and protected the animals from Lassa virus infection and disease after a single immunization but with different efficacy. more...

Organism:

Macaca fascicularis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22523

71 Samples

Download data: CSV

(Submitter supplied) Rhesus macaques (Macaca mulatta) infected with a lethal dose of lymphocytic choriomeningitis virus-strain WE (LCMV-WE) provide a model for Lassa fever virus infection of man. Like Lassa fever in human beings, disease begins with flu-like symptoms but can progress to morbidity fairly rapidly. Previously, we profiled the blood transcriptome of LCMV-infected monkeys (M. Djavani et al. J. Virol. 2007: PMID 17522210) showing distinct pre-viremic and viremic stages that discriminated between virulent and benign infections. more...

Organism:

Macaca mulatta; Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4161

Platform:

GPL570

20 Samples

Download data: CEL

Temporal analysis of liver from macaques infected with virulent lymphocytic choriomeningitis virus (LCMV)-WE or with non-virulent LCMV-Armstrong. LCMV-WE-infected macaques develop viral hemorrhagic fever similar to human Lassa fever. Results provide insight into molecular basis of LCMV pathogenesis.

Organism:

Macaca mulatta; Homo sapiens

Type:

Expression profiling by array, transformed count, 3 disease state, 4 infection, 11 time sets

Platform:

GPL570

Series:

GSE12254

20 Samples

Download data: CEL

(Submitter supplied) Smallpox has played an unparalleled role in human history and remains a significant potential threat to public health. Despite the historical significance of this disease, we know little about the underlying pathophysiology or the virulence mechanisms of the causative agent, variola virus. To improve our understanding of variola pathogenesis and variola-host interactions, we examined the molecular and cellular features of hemorrhagic smallpox in cynomolgus macaques. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL3347 GPL3093 GPL3346

167 Samples

Download data

(Submitter supplied) Lassa fever outbreaks hit West African countries every year and there is still no licensed vaccine to limit the burden of this viral hemorrhagic fever. We previously developed MeV-NP, a single-shot vaccine that induces protective immunity in cynomolgus monkeys one month or more than a year before Lassa virus infection and that is able to protect against divergent viral strains. Given the limited dissemination area of Lassa virus during outbreaks and the high risk of nosocomial transmission, a vaccine that induces rapid protection could be useful to protect exposed people during outbreaks in the absence of preventive vaccination. more...

Organism:

Macaca fascicularis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22523

51 Samples

Download data: CSV

(Submitter supplied) Ebola virus disease (EVD) is a serious illness associated with 20-90% fatalities. EVD is characterized by robust virus replication and strong host inflammatory immune response. Analyzing the host immune response has increasingly involved multimodal approaches including transcriptomics to profile gene expression. We studied cynomolgus macaques exposed to Ebola virus (EBOV) Makona via different routes with the intent of comparing RNA-Seq to a NanoString nCounter codeset targeting 769 non-human primate (NHP) genes. more...

Organism:

Macaca fascicularis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20302

21 Samples

Download data: TXT

(Submitter supplied) Identification of the genetic events that contribute to host-pathogen interactions is important for understanding the natural history of infectious diseases and developing therapeutics. Transcriptome studies conducted on pathogens have been central to this goal in recent years. However, most of these investigations have focused on specific end points or disease phases, rather than analysis of the entire time course of infection. more...

Organism:

Borreliella burgdorferi; Chlamydia trachomatis; Staphylococcus aureus; Coxiella burnetii; Streptococcus pyogenes; Yersinia pestis; Macaca fascicularis

Type:

Expression profiling by array

Platform:

GPL2129

259 Samples

Download data: CEL, EXP, TXT