GEO DataSets

(Submitter supplied) During embryonic development, the neural crest is a transient, migratory cell population that differentiates into a large variety of tissues and contributes much to the formation of vertebrate body. In Xenopus, lrig3 is involved in neural crest formation by modulating FGF and Wnt signaling. Lrig3 functions downstream of pax3 and zic1 to regulate the expression of neural crest markers. We used microarrays to identify the lrig3 target genes during neural crest formation.

Organism:

Xenopus laevis

Type:

Expression profiling by array

Platform:

GPL10756

8 Samples

Download data: CEL, CHP

(Submitter supplied) The cement gland in Xenopus laevis has long been used as a model to study the interplay of cell signaling and transcription factors during embryogenesis. The homeodomain-containing transcription factor Pitx1 has been linked to cement gland development. However, the downstream transcriptional targets of Pitx1 remain unknown. Here, we utilize RNA Sequencing to identify transcripts whose expression are affected by Pitx1 misexpression in animal cap explants.

Organism:

Xenopus laevis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18936

4 Samples

Download data: XLSX

(Submitter supplied) The transcription factors Pax3 and Zic1 are among the earliest genes activated at the neural plate border. Pax3 and Zic1 in combination promote neural crest fate, while Zic1 alone regulate cranial placode progenitor formation. We used microarrays to identify the global repertoire of genes activated by these facors individually or in combination to gain insights into the molecular mechanisms underlying cell fate decision at the neural plate border.

Organism:

Xenopus laevis

Type:

Expression profiling by array

Platform:

GPL10756

20 Samples

Download data: CEL

(Submitter supplied) Exostosin 1 (Ext1) is a glycosyltransferase involved in the biosynthesis of the extracellular matrix Heparan Sulfate Proteoglycan (HSPG). Knockdown of Ext1 caused gastrulation defects and formation of an abnormal body axis. Since ext1 has been implicated as an indirect contributor to multiple signaling pathways in vertebrate development, microarray was used to identify genes expressed in gastrulae that would be affected by a reduction in ext1 expression. more...

Organism:

Xenopus laevis

Type:

Expression profiling by array

Platform:

GPL10756

6 Samples

Download data: CEL

(Submitter supplied) We aimed to identify genes that are inhibited by Kctd15 overexpression during neural crest (NC) induction. Injection of RNAs encoding Wnt3a and chordin into the embryo followed by animal cap explant culture leads to the induction of NC marker genes as well as additional genes expressed in the neural plate border. Our previous study indicated that co-injection of kctd15 inhibits the induction of these markers. more...

Organism:

Xenopus laevis

Type:

Expression profiling by array

Platform:

GPL10756

12 Samples

Download data: CEL, CHP

(Submitter supplied) Studies on the early embryonic development of Xenopus laevis contributed much to the understanding of vertebrate patterning. Gastrula stages are of particular interest because establishment of the axis and germ layer formation take place during these stages. While many genes belonging to several signaling pathways including FGF, Wnt and TGF-beta, have been implicated in patterning the gastrula embryo, the hierarchical interactions between these factors are incompletely known. more...

Organism:

Xenopus laevis

Type:

Expression profiling by array

Platform:

GPL1318

4 Samples

Download data: CEL, CHP

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Xenopus laevis

Type:

Expression profiling by array

Platforms:

GPL10756 GPL1318

27 Samples

Download data: CEL

(Submitter supplied) Neural crest development is orchestrated by a complex and still poorly understood gene regulatory network. Premigratory neural crest is induced at the lateral border of the neural plate by the combined action of signaling molecules and transcription factors such as AP2, Gbx2, Pax3 and Zic1. Among them, Pax3 and Zic1 are both necessary and sufficient to trigger a complete neural crest developmental program. more...

Organism:

Xenopus laevis

Type:

Expression profiling by array

Platform:

GPL1318

11 Samples

Download data: CEL

(Submitter supplied) Neural crest development is orchestrated by a complex and still poorly understood gene regulatory network. Premigratory neural crest is induced at the lateral border of the neural plate by the combined action of signaling molecules and transcription factors such as AP2, Gbx2, Pax3 and Zic1. Among them, Pax3 and Zic1 are both necessary and sufficient to trigger a complete neural crest developmental program. more...

Organism:

Xenopus laevis

Type:

Expression profiling by array

Platform:

GPL10756

16 Samples

Download data: CEL

(Submitter supplied) In order to isolate novel genes regulating neural induction, we utilized a DNA microarray approach. As neural induction is thought to occur via the inhibition of BMP signaling, BMP signaling was inhibited in ectodermal cells by overexpression of a dominant-negative receptor. RNAs were isolated from control animal cap explants and from dominant-negative BMP receptor expressing animal caps and subjected to a microarray experiment using newly generated high-density Xenopus DNA microarray chips. more...

Organism:

Xenopus laevis

Type:

Expression profiling by array

Platforms:

GPL1861 GPL1860

4 Samples

Download data

(Submitter supplied) This microarray was created using normalized cDNA libraries. These libraries were generated using tailbud Xenopus embryos. The EST sequence information of arrayed cDNAs is available at the XDB website, http://xenopus.nibb.ac.jp. Amplified cDNAs from libraries were spotted on the glass slides. 21504 spots. Keywords = Xenopus laevis Keywords = neurula Keywords = tailbud Keywords = cDNA

Organism:

Xenopus laevis

3 Series

6 Samples

Download data

(Submitter supplied) This microarray was created using normalized cDNA libraries. These libraries were generated using neurula and tailbud Xenopus embryos. The EST sequence information of arrayed cDNAs is available at the XDB website, http://xenopus.nibb.ac.jp. Amplified cDNAs from libraries were spotted on the glass slides. 21504 spots. Keywords = Xenopus laevis Keywords = neurula Keywords = tailbud Keywords = cDNA

Organism:

Xenopus laevis

3 Series

6 Samples

Download data

(Submitter supplied) Foregut organogenesis is regulated by inductive interactions between the endoderm and the adjacent mesoderm. We identified genes induced in the foregut progenitors by the adjacent mesoderm. We used microarrays to detail the global programme of early foregut endoderm gene expression resulting from mesoderm induction and identified distinct classes of up-regulated genes during this process.

Organism:

Xenopus laevis

Type:

Expression profiling by array

Platform:

GPL1318

8 Samples

Download data: CEL, TXT

(Submitter supplied) ADAMs are transmembrane metalloproteases that control cell behavior by cleaving both cell adhesion and signaling molecules. The cytoplasmic domain of ADAMs can regulate the proteolytic activity by controlling the subcellular localization and/or the activation of the protease domain. Here we show that the cytoplasmic domain of ADAM13 is cleaved and translocates into the nucleus. Preventing this translocation renders the protein incapable of promoting cranial neural crest (CNC) cell migration in vivo, without affecting its proteolytic activity. more...

Organism:

Xenopus laevis

Type:

Expression profiling by array

Platform:

GPL1318

9 Samples

Download data: CEL, TXT

(Submitter supplied) Analysis of epithelial explants injected with the intracellular domain of Notch (ICD) to block the formation of multi-ciliate and proton secreting cells or with dominant negative human Mastermind (HMM) or a DNA binding mutant of Mastermind (DBM) to induce the formation of ectopic multi-ciliate and proton secreting cells. Results show which genes are up or down-regulated when DBM/HMM are compared to ICD. more...

Organism:

Xenopus laevis

Type:

Expression profiling by array

Platform:

GPL10756

4 Samples

Download data: CEL

(Submitter supplied) Surgical removal of the lens from larval Xenopus laevis results in a rapid transdifferention of central corneal cells to form a new lens. The trigger for this process is understood to be an induction event arising from the unprecedented contact between the cornea and the vitreous humour that occurs following lens removal. The identity of this trigger is unknown. Here, we have used a functional transgenic approach to show that BMP signalling is required for lens regeneration and a microarray approach to identify genes that are upregulated specifically during this process. more...

Organism:

Xenopus laevis

Type:

Expression profiling by array

Platform:

GPL1318

9 Samples

Download data: CEL

(Submitter supplied) sox10:GFP transgenic embryos were used to FACS sort neural crest and microarray analysis was done to compare genes expressed differentially between GFP positive and negative and fold changes were compared to differentially expressed genes in embryos treated with morpholino oligonucleotides to knockdown function of Twist1

Organism:

Danio rerio

Type:

Expression profiling by array

Platform:

GPL14607

14 Samples

Download data: CALLS, PAIR

(Submitter supplied) Bone Morphogenetic Proteins (BMPs), a subgroup of the TGF-beta superfamily, were originally isolated from bone on the basis of their ability to induce ectopic bone development. While BMPs are involved in a wide range of developmental and physiological functions, very few vertebrate target genes in this pathway have been identified. To identify target genes regulated by the BMP growth factor family in Xenopus, large-scale microarray analyses were conducted to discover genes directly activated by this factor in dissociated animal cap tissues treated with a combination of the protein synthesis inhibitor cycloheximide and BMP2. more...

Organism:

Xenopus laevis

Type:

Expression profiling by array

Platforms:

GPL1860 GPL1861

2 Samples

Download data

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Xenopus laevis

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL21248

12 Samples

Download data

(Submitter supplied) VEGFA signaling is crucial for physiological and pathological angiogenesis and hematopoiesis. Although many context-dependent signaling pathways downstream of VegfA have been uncovered, vegfa transcriptional regulation in vivo is unclear. Here, we show that ETS transcription factor Etv6 positively regulates vegfa expression during blood stem cell development through multiple transcriptional inputs. more...

Organism:

Xenopus laevis

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL21248

6 Samples

Download data: BED