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| Status |
Public on Sep 14, 2011 |
| Title |
Identification of genes associated with lens regeneration from the cornea in Xenopus laevis tadpoles |
| Organism |
Xenopus laevis |
| Experiment type |
Expression profiling by array
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| Summary |
Surgical removal of the lens from larval Xenopus laevis results in a rapid transdifferention of central corneal cells to form a new lens. The trigger for this process is understood to be an induction event arising from the unprecedented contact between the cornea and the vitreous humour that occurs following lens removal. The identity of this trigger is unknown. Here, we have used a functional transgenic approach to show that BMP signalling is required for lens regeneration and a microarray approach to identify genes that are upregulated specifically during this process. Analysis of the array data strongly implicates Wnt signalling and Pitx transcription factors in this process. Pluripotency genes, in contrast, are not upregulated, supporting the idea that corneal cells transdifferentiate without returning to a stem cell state. Furthermore, several genes from the array were expressed in the forming lens during embryogenesis. One of these, nipsnap1, is a known direct target of BMP signalling. We suggest that, as with tail regeneration, activation of multiple developmental signalling pathways could drive lens regeneration from the cornea.
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| Overall design |
Three biological replicates of each of three sample types were analysed. For each replicate, lens tissue (L) was derived from 5 lenses dissected from stage 50 tadpoles. Similarly, 7-8 corneas dissected from stage 50 tadpoles from which the lens had been removed 3 days previously were pooled for each biologocal replicate (R). These samples should contain tissue that is changing from cornea to lens, to regenerate the missing lens. Finally, 7-8 corneas from eyes of tadpoles at the same stage that had the cornea lifetd but the lens left in place 3 days earlier were pooled for each biological replicate (sham operated corneas, designated S).
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| Contributor(s) |
Beck CW, Day RC |
| Citation(s) |
21896182 |
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| Submission date |
Mar 17, 2011 |
| Last update date |
Mar 23, 2012 |
| Contact name |
Caroline Beck |
| E-mail(s) |
caroline.beck@otago.ac.nz
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| Organization name |
University of Otago
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| Department |
Zoology
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| Street address |
340 Great King Street
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| City |
Dunedin |
| ZIP/Postal code |
9054 |
| Country |
New Zealand |
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| Platforms (1) |
| GPL1318 |
[Xenopus_laevis] Affymetrix Xenopus laevis Genome Array |
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| Samples (9)
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| GSM692818 |
R1: corneas dissected 3 days after removal of lens at stage 50 |
| GSM692819 |
R2: corneas dissected 3 days after removal of lens at stage 50 |
| GSM692820 |
R3: corneas dissected 3 days after removal of lens at stage 50 |
| GSM692821 |
S1: corneas dissected 3 days after sham operated (lens left in) at stage 50 |
| GSM692822 |
S3: corneas dissected 3 days after sham operated (lens left in) at stage 50 |
| GSM692823 |
S4: corneas dissected 3 days after sham operated (lens left in) at stage 50 |
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| Relations |
| BioProject |
PRJNA137989 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE28014_RAW.tar |
12.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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